A rapid procedure for isolating mitochondrial DNA

Earl G. Zimmerman, Darrin R. Akins, John V. Planz, Michael J. Schurr

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


A technique for the rapid isolation of mitochondrial DNA (mtDNA) from animal tissues is described that eliminates the time-consuming separation of nuclear and mtDNAs using cesium chloride gradient ultracentrifugation. The procedure utilizes digestion of the nuclear DNA with DNase, after which lysis of mitochondria and subsequent extraction of proteins results in relatively pure mtDNA. Up to 5 μg of mtDNA per gram of liver tissue resulted, a suitable yield for five digests with restriction enzymes and staining with ethidium bromide.

Original languageEnglish
Pages (from-to)102-104
Number of pages3
JournalGene Analysis Techniques
Issue number5
StatePublished - 1988


Dive into the research topics of 'A rapid procedure for isolating mitochondrial DNA'. Together they form a unique fingerprint.

Cite this