A rapid procedure for isolating mitochondrial DNA

Earl G. Zimmerman; Darrin R. Akins; John V. Planz; Michael J. Schurr

doi:10.1016/0735-0651(88)90004-0

A rapid procedure for isolating mitochondrial DNA

Earl G. Zimmerman, Darrin R. Akins, John V. Planz, Michael J. Schurr

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A technique for the rapid isolation of mitochondrial DNA (mtDNA) from animal tissues is described that eliminates the time-consuming separation of nuclear and mtDNAs using cesium chloride gradient ultracentrifugation. The procedure utilizes digestion of the nuclear DNA with DNase, after which lysis of mitochondria and subsequent extraction of proteins results in relatively pure mtDNA. Up to 5 μg of mtDNA per gram of liver tissue resulted, a suitable yield for five digests with restriction enzymes and staining with ethidium bromide.

Original language	English
Pages (from-to)	102-104
Number of pages	3
Journal	Gene Analysis Techniques
Volume	5
Issue number	5
DOIs	https://doi.org/10.1016/0735-0651(88)90004-0
State	Published - 1988

Access to Document

10.1016/0735-0651(88)90004-0

Cite this

@article{c203d3999c034764b9894cdf24fde6e8,

title = "A rapid procedure for isolating mitochondrial DNA",

abstract = "A technique for the rapid isolation of mitochondrial DNA (mtDNA) from animal tissues is described that eliminates the time-consuming separation of nuclear and mtDNAs using cesium chloride gradient ultracentrifugation. The procedure utilizes digestion of the nuclear DNA with DNase, after which lysis of mitochondria and subsequent extraction of proteins results in relatively pure mtDNA. Up to 5 μg of mtDNA per gram of liver tissue resulted, a suitable yield for five digests with restriction enzymes and staining with ethidium bromide.",

author = "Zimmerman, {Earl G.} and Akins, {Darrin R.} and Planz, {John V.} and Schurr, {Michael J.}",

note = "Funding Information: This work was supported by grant 2 S07 RR07195 from the National Institutes of Health Biomedical Research Support Program and a Faculty Research Grant from the University of North Texas.",

year = "1988",

doi = "10.1016/0735-0651(88)90004-0",

language = "English",

volume = "5",

pages = "102--104",

journal = "Gene Analysis Techniques",

issn = "0735-0651",

publisher = "Elsevier BV",

number = "5",

}

TY - JOUR

T1 - A rapid procedure for isolating mitochondrial DNA

AU - Zimmerman, Earl G.

AU - Akins, Darrin R.

AU - Planz, John V.

AU - Schurr, Michael J.

N1 - Funding Information: This work was supported by grant 2 S07 RR07195 from the National Institutes of Health Biomedical Research Support Program and a Faculty Research Grant from the University of North Texas.

PY - 1988

Y1 - 1988

N2 - A technique for the rapid isolation of mitochondrial DNA (mtDNA) from animal tissues is described that eliminates the time-consuming separation of nuclear and mtDNAs using cesium chloride gradient ultracentrifugation. The procedure utilizes digestion of the nuclear DNA with DNase, after which lysis of mitochondria and subsequent extraction of proteins results in relatively pure mtDNA. Up to 5 μg of mtDNA per gram of liver tissue resulted, a suitable yield for five digests with restriction enzymes and staining with ethidium bromide.

AB - A technique for the rapid isolation of mitochondrial DNA (mtDNA) from animal tissues is described that eliminates the time-consuming separation of nuclear and mtDNAs using cesium chloride gradient ultracentrifugation. The procedure utilizes digestion of the nuclear DNA with DNase, after which lysis of mitochondria and subsequent extraction of proteins results in relatively pure mtDNA. Up to 5 μg of mtDNA per gram of liver tissue resulted, a suitable yield for five digests with restriction enzymes and staining with ethidium bromide.

UR - http://www.scopus.com/inward/record.url?scp=0023795283&partnerID=8YFLogxK

U2 - 10.1016/0735-0651(88)90004-0

DO - 10.1016/0735-0651(88)90004-0

M3 - Article

C2 - 2847966

AN - SCOPUS:0023795283

SN - 0735-0651

VL - 5

SP - 102

EP - 104

JO - Gene Analysis Techniques

JF - Gene Analysis Techniques

IS - 5

ER -

A rapid procedure for isolating mitochondrial DNA

Abstract

Access to Document

Other files and links

Fingerprint

Cite this