A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolus carbonum

Yi Qiang Cheng; Joong Hoon Ahn; Jonathan D. Walton

doi:10.1099/00221287-145-12-3539

A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolus carbonum

Yi Qiang Cheng, Joong Hoon Ahn, Jonathan D. Walton

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31 Scopus citations

Abstract

The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus Cochliobolus carbonum on maize. HC-toxin production is controlled by a complex locus, TOX2. The isolation and characterization of a new gene of the TOX2 locus, TOXF, is reported. It is shown that TOXF is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2⁺) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of TOXF has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of C. carbonum with all copies of TOXF disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that TOXF has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2-amino-9, 10-epoxi-8-oxodecanoic acid).

Original language	English
Pages (from-to)	3539-3546
Number of pages	8
Journal	Microbiology
Volume	145
Issue number	12
DOIs	https://doi.org/10.1099/00221287-145-12-3539
State	Published - Dec 1999

Keywords

Branched-chain-amino-acid transaminase
Cochliobolus (Helminthosporium) carbonum
Cyclic peptide
HC-toxin
TOXF

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10.1099/00221287-145-12-3539

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title = "A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolus carbonum",

abstract = "The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus Cochliobolus carbonum on maize. HC-toxin production is controlled by a complex locus, TOX2. The isolation and characterization of a new gene of the TOX2 locus, TOXF, is reported. It is shown that TOXF is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2+) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of TOXF has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of C. carbonum with all copies of TOXF disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that TOXF has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2-amino-9, 10-epoxi-8-oxodecanoic acid).",

keywords = "Branched-chain-amino-acid transaminase, Cochliobolus (Helminthosporium) carbonum, Cyclic peptide, HC-toxin, TOXF",

author = "Cheng, {Yi Qiang} and Ahn, {Joong Hoon} and Walton, {Jonathan D.}",

year = "1999",

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AU - Cheng, Yi Qiang

AU - Ahn, Joong Hoon

AU - Walton, Jonathan D.

PY - 1999/12

Y1 - 1999/12

N2 - The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus Cochliobolus carbonum on maize. HC-toxin production is controlled by a complex locus, TOX2. The isolation and characterization of a new gene of the TOX2 locus, TOXF, is reported. It is shown that TOXF is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2+) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of TOXF has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of C. carbonum with all copies of TOXF disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that TOXF has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2-amino-9, 10-epoxi-8-oxodecanoic acid).

AB - The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus Cochliobolus carbonum on maize. HC-toxin production is controlled by a complex locus, TOX2. The isolation and characterization of a new gene of the TOX2 locus, TOXF, is reported. It is shown that TOXF is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2+) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of TOXF has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of C. carbonum with all copies of TOXF disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that TOXF has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2-amino-9, 10-epoxi-8-oxodecanoic acid).

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KW - Cochliobolus (Helminthosporium) carbonum

KW - Cyclic peptide

KW - HC-toxin

KW - TOXF

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SN - 1350-0872

VL - 145

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JO - Microbiology

JF - Microbiology

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ER -

A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolus carbonum

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