A protein biosensor for lactate

Sabato D’auria, Zygmunt Gryczynski, Ignacy Gryczynski, Mosé Rossi, Joseph R. Lakowicz

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for (L)-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8- anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalAnalytical Biochemistry
Volume283
Issue number1
DOIs
StatePublished - 15 Jul 2000

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Biosensing Techniques
Biosensors
Lactic Acid
L-Lactate Dehydrogenase
Sulfonic Acids
Beef
Proteins
Point-of-Care Systems
Miniaturization
Protein Engineering
Blood
Polarization
naphthalene

Cite this

D’auria, Sabato ; Gryczynski, Zygmunt ; Gryczynski, Ignacy ; Rossi, Mosé ; Lakowicz, Joseph R. / A protein biosensor for lactate. In: Analytical Biochemistry. 2000 ; Vol. 283, No. 1. pp. 83-88.
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A protein biosensor for lactate. / D’auria, Sabato; Gryczynski, Zygmunt; Gryczynski, Ignacy; Rossi, Mosé; Lakowicz, Joseph R.

In: Analytical Biochemistry, Vol. 283, No. 1, 15.07.2000, p. 83-88.

Research output: Contribution to journalArticle

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