A protein biosensor for lactate

Sabato D’auria; Zygmunt Gryczynski; Ignacy Gryczynski; Mosé Rossi; Joseph R. Lakowicz

doi:10.1006/abio.2000.4611

A protein biosensor for lactate

Sabato D’auria, Zygmunt Gryczynski, Ignacy Gryczynski, Mosé Rossi, Joseph R. Lakowicz

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for (L)-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8- anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	83-88
Number of pages	6
Journal	Analytical Biochemistry
Volume	283
Issue number	1
DOIs	https://doi.org/10.1006/abio.2000.4611
State	Published - 15 Jul 2000

Access to Document

10.1006/abio.2000.4611

Cite this

@article{afa083ba5a0a4d93b01112acb42c395f,

title = "A protein biosensor for lactate",

abstract = "Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for (L)-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8- anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein. (C) 2000 Academic Press.",

author = "Sabato D{\textquoteright}auria and Zygmunt Gryczynski and Ignacy Gryczynski and Mos{\'e} Rossi and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by the National Institutes of Health, National Center for Research Resources, RR-08119 and by an EU contract on Extremeophiles to Professor Mos{\'e} Rossi.",

year = "2000",

month = jul,

day = "15",

doi = "10.1006/abio.2000.4611",

language = "English",

volume = "283",

pages = "83--88",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A protein biosensor for lactate

AU - D’auria, Sabato

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

AU - Rossi, Mosé

AU - Lakowicz, Joseph R.

N1 - Funding Information: This work was supported by the National Institutes of Health, National Center for Research Resources, RR-08119 and by an EU contract on Extremeophiles to Professor Mosé Rossi.

PY - 2000/7/15

Y1 - 2000/7/15

N2 - Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for (L)-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8- anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein. (C) 2000 Academic Press.

AB - Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for (L)-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8- anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein. (C) 2000 Academic Press.

UR - http://www.scopus.com/inward/record.url?scp=0034662256&partnerID=8YFLogxK

U2 - 10.1006/abio.2000.4611

DO - 10.1006/abio.2000.4611

M3 - Article

C2 - 10929812

AN - SCOPUS:0034662256

SN - 0003-2697

VL - 283

SP - 83

EP - 88

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A protein biosensor for lactate

Abstract

Access to Document

Other files and links

Fingerprint

Cite this