A priori dietary ω-3 lipid supplementation results in local pancreatic macrophage and pulmonary inflammatory response attenuation in a model of experimental acute edematous pancreatitis (AEP)

Suhail Sharif, Michael Broman, Tricia Babcock, Evan Ong, David Jho, Marek Rudnicki, W. Scott Helton, N. Joseph Espat

Research output: Contribution to journalArticle

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Abstract

Background: Acute pancreatitis is often complicated by multiorgan dysfunction, which is postulated to occur in part by macrophage infiltration into the pancreas. Eicosa-pentaenoic acid (EPA), an ω-3 fatty acid, is the principal biologic component of fish oil and has clinically and experimentally been demonstrated to be anti-inflammatory. We hypothesized that dietary EPA supplementation before the induction of pancreatitis would attenuate both MΦ-mediated local pancreatic and systemic pulmonary inflammatory response in an in vivo model of acute edematous pancreatitis (AEP). Methods: Male Sprague-Dawley (SD) rats were pre-treated 2 times per day with oral gavage with EPA (ω-3 fatty acid; 5 mg/kg/dose) or ω-6 fatty acid control (5 mg/kg/dose) or saline (equal volume) for 2 weeks. AEP was induced in ω-3, w-6, and saline pretreated rats by 5 hourly subcutaneous (SC) injections of cerulein. Pancreas, lung, and serum were harvested 3 hours after the last cerulein injection. Severity of Correspondence: N. Joseph Espat, MD, MS, FACS, Associate Professor of Surgery, University of Illinois at Chicago, Department of Surgery M/C 958, 840 S. Wood St, Room 435E, Chicago, IL 60612. pancreatitis was confirmed by serum amylase and by histopathologic score. Pancreatic macrophage infiltration was assessed by confocal fluorescent microscopy, and pulmonary leukocyte respiratory burst (LRB) analysis was performed on mononuclear cells obtained from bronchioalveolar lavage (BAL). Results: All animals demonstrated acute pancreatitis through hyperamylasemia and histopathologic examination. Confocal analysis demonstrated significantly lower macrophage infiltration, and BAL analysis by flow cytometry demonstrated significantly lower (p < .05) LRB in the ω-3-treated group compared with the ω-6 and the saline pancreatitis group. Conclusions: Attenuation of both pancreatic MΦ inflammatory response and pulmonary leukocyte respiratory burst in AEP by EPA supports further investigation into the potential role for EPA dietary supplementation in the progression of pancreatitis-rassociated sequelae. (Journal of Parenteral and Enteral Nutrition 30:271-276, 2006)

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalJournal of Parenteral and Enteral Nutrition
Volume30
Issue number4
DOIs
StatePublished - 1 Dec 2006

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pancreatitis
Alveolar Macrophages
Pancreatitis
macrophages
Theoretical Models
inflammation
lungs
Lipids
lipids
Respiratory Burst
Acids
acids
Ceruletide
leukocytes
Lung
Leukocytes
Fatty Acids
Macrophages
Bronchoalveolar Lavage
fatty acids

Cite this

Sharif, Suhail ; Broman, Michael ; Babcock, Tricia ; Ong, Evan ; Jho, David ; Rudnicki, Marek ; Helton, W. Scott ; Espat, N. Joseph. / A priori dietary ω-3 lipid supplementation results in local pancreatic macrophage and pulmonary inflammatory response attenuation in a model of experimental acute edematous pancreatitis (AEP). In: Journal of Parenteral and Enteral Nutrition. 2006 ; Vol. 30, No. 4. pp. 271-276.
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abstract = "Background: Acute pancreatitis is often complicated by multiorgan dysfunction, which is postulated to occur in part by macrophage infiltration into the pancreas. Eicosa-pentaenoic acid (EPA), an ω-3 fatty acid, is the principal biologic component of fish oil and has clinically and experimentally been demonstrated to be anti-inflammatory. We hypothesized that dietary EPA supplementation before the induction of pancreatitis would attenuate both MΦ-mediated local pancreatic and systemic pulmonary inflammatory response in an in vivo model of acute edematous pancreatitis (AEP). Methods: Male Sprague-Dawley (SD) rats were pre-treated 2 times per day with oral gavage with EPA (ω-3 fatty acid; 5 mg/kg/dose) or ω-6 fatty acid control (5 mg/kg/dose) or saline (equal volume) for 2 weeks. AEP was induced in ω-3, w-6, and saline pretreated rats by 5 hourly subcutaneous (SC) injections of cerulein. Pancreas, lung, and serum were harvested 3 hours after the last cerulein injection. Severity of Correspondence: N. Joseph Espat, MD, MS, FACS, Associate Professor of Surgery, University of Illinois at Chicago, Department of Surgery M/C 958, 840 S. Wood St, Room 435E, Chicago, IL 60612. pancreatitis was confirmed by serum amylase and by histopathologic score. Pancreatic macrophage infiltration was assessed by confocal fluorescent microscopy, and pulmonary leukocyte respiratory burst (LRB) analysis was performed on mononuclear cells obtained from bronchioalveolar lavage (BAL). Results: All animals demonstrated acute pancreatitis through hyperamylasemia and histopathologic examination. Confocal analysis demonstrated significantly lower macrophage infiltration, and BAL analysis by flow cytometry demonstrated significantly lower (p < .05) LRB in the ω-3-treated group compared with the ω-6 and the saline pancreatitis group. Conclusions: Attenuation of both pancreatic MΦ inflammatory response and pulmonary leukocyte respiratory burst in AEP by EPA supports further investigation into the potential role for EPA dietary supplementation in the progression of pancreatitis-rassociated sequelae. (Journal of Parenteral and Enteral Nutrition 30:271-276, 2006)",
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A priori dietary ω-3 lipid supplementation results in local pancreatic macrophage and pulmonary inflammatory response attenuation in a model of experimental acute edematous pancreatitis (AEP). / Sharif, Suhail; Broman, Michael; Babcock, Tricia; Ong, Evan; Jho, David; Rudnicki, Marek; Helton, W. Scott; Espat, N. Joseph.

In: Journal of Parenteral and Enteral Nutrition, Vol. 30, No. 4, 01.12.2006, p. 271-276.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A priori dietary ω-3 lipid supplementation results in local pancreatic macrophage and pulmonary inflammatory response attenuation in a model of experimental acute edematous pancreatitis (AEP)

AU - Sharif, Suhail

AU - Broman, Michael

AU - Babcock, Tricia

AU - Ong, Evan

AU - Jho, David

AU - Rudnicki, Marek

AU - Helton, W. Scott

AU - Espat, N. Joseph

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Background: Acute pancreatitis is often complicated by multiorgan dysfunction, which is postulated to occur in part by macrophage infiltration into the pancreas. Eicosa-pentaenoic acid (EPA), an ω-3 fatty acid, is the principal biologic component of fish oil and has clinically and experimentally been demonstrated to be anti-inflammatory. We hypothesized that dietary EPA supplementation before the induction of pancreatitis would attenuate both MΦ-mediated local pancreatic and systemic pulmonary inflammatory response in an in vivo model of acute edematous pancreatitis (AEP). Methods: Male Sprague-Dawley (SD) rats were pre-treated 2 times per day with oral gavage with EPA (ω-3 fatty acid; 5 mg/kg/dose) or ω-6 fatty acid control (5 mg/kg/dose) or saline (equal volume) for 2 weeks. AEP was induced in ω-3, w-6, and saline pretreated rats by 5 hourly subcutaneous (SC) injections of cerulein. Pancreas, lung, and serum were harvested 3 hours after the last cerulein injection. Severity of Correspondence: N. Joseph Espat, MD, MS, FACS, Associate Professor of Surgery, University of Illinois at Chicago, Department of Surgery M/C 958, 840 S. Wood St, Room 435E, Chicago, IL 60612. pancreatitis was confirmed by serum amylase and by histopathologic score. Pancreatic macrophage infiltration was assessed by confocal fluorescent microscopy, and pulmonary leukocyte respiratory burst (LRB) analysis was performed on mononuclear cells obtained from bronchioalveolar lavage (BAL). Results: All animals demonstrated acute pancreatitis through hyperamylasemia and histopathologic examination. Confocal analysis demonstrated significantly lower macrophage infiltration, and BAL analysis by flow cytometry demonstrated significantly lower (p < .05) LRB in the ω-3-treated group compared with the ω-6 and the saline pancreatitis group. Conclusions: Attenuation of both pancreatic MΦ inflammatory response and pulmonary leukocyte respiratory burst in AEP by EPA supports further investigation into the potential role for EPA dietary supplementation in the progression of pancreatitis-rassociated sequelae. (Journal of Parenteral and Enteral Nutrition 30:271-276, 2006)

AB - Background: Acute pancreatitis is often complicated by multiorgan dysfunction, which is postulated to occur in part by macrophage infiltration into the pancreas. Eicosa-pentaenoic acid (EPA), an ω-3 fatty acid, is the principal biologic component of fish oil and has clinically and experimentally been demonstrated to be anti-inflammatory. We hypothesized that dietary EPA supplementation before the induction of pancreatitis would attenuate both MΦ-mediated local pancreatic and systemic pulmonary inflammatory response in an in vivo model of acute edematous pancreatitis (AEP). Methods: Male Sprague-Dawley (SD) rats were pre-treated 2 times per day with oral gavage with EPA (ω-3 fatty acid; 5 mg/kg/dose) or ω-6 fatty acid control (5 mg/kg/dose) or saline (equal volume) for 2 weeks. AEP was induced in ω-3, w-6, and saline pretreated rats by 5 hourly subcutaneous (SC) injections of cerulein. Pancreas, lung, and serum were harvested 3 hours after the last cerulein injection. Severity of Correspondence: N. Joseph Espat, MD, MS, FACS, Associate Professor of Surgery, University of Illinois at Chicago, Department of Surgery M/C 958, 840 S. Wood St, Room 435E, Chicago, IL 60612. pancreatitis was confirmed by serum amylase and by histopathologic score. Pancreatic macrophage infiltration was assessed by confocal fluorescent microscopy, and pulmonary leukocyte respiratory burst (LRB) analysis was performed on mononuclear cells obtained from bronchioalveolar lavage (BAL). Results: All animals demonstrated acute pancreatitis through hyperamylasemia and histopathologic examination. Confocal analysis demonstrated significantly lower macrophage infiltration, and BAL analysis by flow cytometry demonstrated significantly lower (p < .05) LRB in the ω-3-treated group compared with the ω-6 and the saline pancreatitis group. Conclusions: Attenuation of both pancreatic MΦ inflammatory response and pulmonary leukocyte respiratory burst in AEP by EPA supports further investigation into the potential role for EPA dietary supplementation in the progression of pancreatitis-rassociated sequelae. (Journal of Parenteral and Enteral Nutrition 30:271-276, 2006)

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