A persistent sodium current in acutely isolated histaminergic neurons from rat hypothalamus

V. Uteshev; D. R. Stevens; H. L. Haas

doi:10.1016/0306-4522(94)00593-T

A persistent sodium current in acutely isolated histaminergic neurons from rat hypothalamus

V. Uteshev, D. R. Stevens, H. L. Haas

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Abstract

Histamine neurons acutely dissociated from the tuberomammillary nucleus of the rat hypothalamus were studied in whole-cell and cell-attached patch-clamp experiments. Electrophysiological properties of dissociated cells were found to be similar to those recorded in slice experiments using microelectrodes. Tuberomammillary neurons fired spontaneously and this activity persisted when Cs⁺ (1.5 mM) was added to, or when K⁺ was removed from the extracellular solution. In whole-cell experiments a persistent tetrodotoxin-sensitive inward current was recorded. In cell attached recordings voltage-gated sodium channels displayed either normal or non-inactivating behavior. These results provide a further analysis of the properties of histaminergic neurons and indicate that spontaneous activity is intrinsic to individual neurons. Evidence for a non-inactivating tetrodotoxin-sensitive sodium current is presented. Single channel recordings indicate that this current is the result of non-inactivating behavior of sodium channels. Such a current is well suited for biasing tuberomammillary neurons toward spontaneous activity.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Neuroscience
Volume	66
Issue number	1
DOIs	https://doi.org/10.1016/0306-4522(94)00593-T
State	Published - May 1995

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@article{064a7db220b64902906b72733dce7b6e,

title = "A persistent sodium current in acutely isolated histaminergic neurons from rat hypothalamus",

abstract = "Histamine neurons acutely dissociated from the tuberomammillary nucleus of the rat hypothalamus were studied in whole-cell and cell-attached patch-clamp experiments. Electrophysiological properties of dissociated cells were found to be similar to those recorded in slice experiments using microelectrodes. Tuberomammillary neurons fired spontaneously and this activity persisted when Cs+ (1.5 mM) was added to, or when K+ was removed from the extracellular solution. In whole-cell experiments a persistent tetrodotoxin-sensitive inward current was recorded. In cell attached recordings voltage-gated sodium channels displayed either normal or non-inactivating behavior. These results provide a further analysis of the properties of histaminergic neurons and indicate that spontaneous activity is intrinsic to individual neurons. Evidence for a non-inactivating tetrodotoxin-sensitive sodium current is presented. Single channel recordings indicate that this current is the result of non-inactivating behavior of sodium channels. Such a current is well suited for biasing tuberomammillary neurons toward spontaneous activity.",

author = "V. Uteshev and Stevens, {D. R.} and Haas, {H. L.}",

note = "Funding Information: These results demonstrate the presence of non-activating sodium channels in isolated TM neurons. These channels may contribute a small inward current at resting potential and could contribute to action potential generation following recovery from the afterhyperpolarization. Since spontaneous openings in the absence of depolarization were not seen, it is likely that the major action of this current is to enhance depolarizing events. 2° Acknowledgements--This work was supported by DFG 1525/1-2 and BMFZ D~isseldorf. We thank Dr J. Janovsky for helpful discussions.",

year = "1995",

month = may,

doi = "10.1016/0306-4522(94)00593-T",

language = "English",

volume = "66",

pages = "143--149",

journal = "Neuroscience",

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T1 - A persistent sodium current in acutely isolated histaminergic neurons from rat hypothalamus

AU - Uteshev, V.

AU - Stevens, D. R.

AU - Haas, H. L.

N1 - Funding Information: These results demonstrate the presence of non-activating sodium channels in isolated TM neurons. These channels may contribute a small inward current at resting potential and could contribute to action potential generation following recovery from the afterhyperpolarization. Since spontaneous openings in the absence of depolarization were not seen, it is likely that the major action of this current is to enhance depolarizing events. 2° Acknowledgements--This work was supported by DFG 1525/1-2 and BMFZ D~isseldorf. We thank Dr J. Janovsky for helpful discussions.

PY - 1995/5

Y1 - 1995/5

N2 - Histamine neurons acutely dissociated from the tuberomammillary nucleus of the rat hypothalamus were studied in whole-cell and cell-attached patch-clamp experiments. Electrophysiological properties of dissociated cells were found to be similar to those recorded in slice experiments using microelectrodes. Tuberomammillary neurons fired spontaneously and this activity persisted when Cs+ (1.5 mM) was added to, or when K+ was removed from the extracellular solution. In whole-cell experiments a persistent tetrodotoxin-sensitive inward current was recorded. In cell attached recordings voltage-gated sodium channels displayed either normal or non-inactivating behavior. These results provide a further analysis of the properties of histaminergic neurons and indicate that spontaneous activity is intrinsic to individual neurons. Evidence for a non-inactivating tetrodotoxin-sensitive sodium current is presented. Single channel recordings indicate that this current is the result of non-inactivating behavior of sodium channels. Such a current is well suited for biasing tuberomammillary neurons toward spontaneous activity.

AB - Histamine neurons acutely dissociated from the tuberomammillary nucleus of the rat hypothalamus were studied in whole-cell and cell-attached patch-clamp experiments. Electrophysiological properties of dissociated cells were found to be similar to those recorded in slice experiments using microelectrodes. Tuberomammillary neurons fired spontaneously and this activity persisted when Cs+ (1.5 mM) was added to, or when K+ was removed from the extracellular solution. In whole-cell experiments a persistent tetrodotoxin-sensitive inward current was recorded. In cell attached recordings voltage-gated sodium channels displayed either normal or non-inactivating behavior. These results provide a further analysis of the properties of histaminergic neurons and indicate that spontaneous activity is intrinsic to individual neurons. Evidence for a non-inactivating tetrodotoxin-sensitive sodium current is presented. Single channel recordings indicate that this current is the result of non-inactivating behavior of sodium channels. Such a current is well suited for biasing tuberomammillary neurons toward spontaneous activity.

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VL - 66

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JO - Neuroscience

JF - Neuroscience

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A persistent sodium current in acutely isolated histaminergic neurons from rat hypothalamus

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