TY - JOUR
T1 - A novel use for coomassie brilliant blue (R-250) in protein gel-drying procedure and assessing the electro-transferring efficiency
AU - Dibas, Adnan I.
AU - Yorio, Thomas
PY - 1996/3/27
Y1 - 1996/3/27
N2 - The present study provides an alternative method for protein-gel drying. Rat brain protein extracts were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with coomassie brilliant blue (R-250). The stained gel was then subjected to electroblotting on nitrocellulose membranes. This method exhibited four advantages: 1) it eliminated problems associated with gel-drying (e.g., shrinkage of gel), 2) it allowed assessment of the efficiency of electro-transfer, 3) it significantly reduced the time of gel-drying procedure by an average of 40 minutes and 4) it facilitated visualizing electro-transferred proteins with the same efficiency as the common amidoblack staining technique. In conclusion, the described method is simple, economical and introduces several applications.
AB - The present study provides an alternative method for protein-gel drying. Rat brain protein extracts were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with coomassie brilliant blue (R-250). The stained gel was then subjected to electroblotting on nitrocellulose membranes. This method exhibited four advantages: 1) it eliminated problems associated with gel-drying (e.g., shrinkage of gel), 2) it allowed assessment of the efficiency of electro-transfer, 3) it significantly reduced the time of gel-drying procedure by an average of 40 minutes and 4) it facilitated visualizing electro-transferred proteins with the same efficiency as the common amidoblack staining technique. In conclusion, the described method is simple, economical and introduces several applications.
UR - http://www.scopus.com/inward/record.url?scp=0029893244&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0508
DO - 10.1006/bbrc.1996.0508
M3 - Article
C2 - 8607869
AN - SCOPUS:0029893244
VL - 220
SP - 929
EP - 932
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -