A Novel Chromatographic Method for the Preparation of High Density Lipoproteins

Mehrnoosh Jahani, Raymond G. Huttash, Andras G. Lacko

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

High density lipoproteins (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. The product was free of low density lipoproteins (LDL) but serum albumin (HSA) was still present. The remaining HSA was removed by an immuno-adsorbent column. The HDL isolated by our method was compared to another HDL preparation isolated from the same plasma sample by the combination of ultracentrifugation and gel chromatography.1 It was found to have approximately the same lipid and protein composition as the HDL isolated by conventional techniques.1 Minor differences included a higher phospholipid and apoprotein E content and lower triglyceride and ApoC II content of the HDL isolated by column chromatography. The method described here is considerably less tedious than earlier techniques, can be scaled up without substantial increase in labor and results in an approximately 30% higher yield than the method described by Rudel et al1.

Original languageEnglish
Pages (from-to)431-444
Number of pages14
JournalPreparative Biochemistry
Volume10
Issue number4
DOIs
StatePublished - 1 Jan 1980

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HDL Lipoproteins
Column chromatography
Chromatography
Apolipoproteins C
Ultracentrifugation
Apolipoproteins E
LDL Lipoproteins
Serum Albumin
Adsorbents
Phospholipids
Triglycerides
Gels
Personnel
Lipids
Plasmas
Chemical analysis
Proteins

Cite this

Jahani, Mehrnoosh ; Huttash, Raymond G. ; Lacko, Andras G. / A Novel Chromatographic Method for the Preparation of High Density Lipoproteins. In: Preparative Biochemistry. 1980 ; Vol. 10, No. 4. pp. 431-444.
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A Novel Chromatographic Method for the Preparation of High Density Lipoproteins. / Jahani, Mehrnoosh; Huttash, Raymond G.; Lacko, Andras G.

In: Preparative Biochemistry, Vol. 10, No. 4, 01.01.1980, p. 431-444.

Research output: Contribution to journalArticle

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