A novel approach for visualization and localization of small amounts of DNA on swabs to improve DNA collection and recovery process

Emma Kitchner, Jose Chavez, Luca Ceresa, Magdalena M. Bus, Bruce Budowle, Zygmunt Gryczynski

Research output: Contribution to journalArticlepeer-review

Abstract

In this report, a simple and practical procedure is proposed for DNA localization on a solid matrix e.g., a collection swab. The approach is straightforward and employs spectrum decomposition using a model DNA intercalator Ethidium Bromide (EtBr). The proposed approach can detect picograms of DNA in solution and nanograms of DNA on solid surfaces (swabs) without the need for PCR amplification. The proposed technology offers the possibility for developing an inexpensive, sensitive, rapid, and practical method for localizing and recovering DNA deposited on collection swabs during routine DNA screening. Improved detection of low DNA concentrations is needed and, if feasible, will allow for better decision making in clinical medicine, biological and environmental research, and human identification in forensic investigations.

Original languageEnglish
Pages (from-to)1198-1206
Number of pages9
JournalAnalyst
Volume146
Issue number4
DOIs
StatePublished - 21 Feb 2021

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