TY - JOUR
T1 - A magnetic immunoconjugate nanoplatform for easy colorimetric detection of the NS1 protein of dengue virus in infected serum
AU - Ramírez-Navarro, Ramsés
AU - Polesnak, Peter
AU - Reyes-Leyva, Julio
AU - Haque, Ubydul
AU - Vazquez-Chagoyán, Juan Carlos
AU - Pedroza-Montero, Martín R.
AU - Méndez-Rojas, Miguel A.
AU - Angulo-Molina, Aracely
N1 - Funding Information:
This work was partially supported by red PRODEP 2016, Project: Nanotechnology as a tool for the Diagnosis and Treatment of Infectious Diseases and by CONACYT (Grants # CB-2010/154602 and INFR-2014/02-23053). Partial support from the Office of Graduate Studies and Research (UDLAP) and CIBIOR is acknowledged.
Funding Information:
The authors are thankful to Adriana Garibay and Alejandro Tapia Gonzalez for their useful suggestions on the manuscript. Technical support of Tania C. Hidalgo-Castillo and Andrea Diaz-Gaxiola (UDLAP), Carolina Jasso and Lilian K. Flores (Laboratory of Immunology and Virology, CIBIOR) and technical support of Biophysics Laboratory-DIFUS, Nanotechnology Laboratory are deeply acknowledged. JRL acknowledges support of Fundación IMSS.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2020/7
Y1 - 2020/7
N2 - In this work, as a proof of principle, the design and performance evaluation of a simple, cheap and efficient colorimetric test for the detection of the NS1 protein of dengue virus, assisted by an immunoconjugate of magnetite (Fe3O4) nanoparticles coupled to anti-NS1 antibodies is reported. A monoclonal antibody against the NS1 antigen was covalently immobilized on the surface of superparamagnetic iron oxide nanoparticles (SPIONs ∼ 20 nm) and used for the immunodetection of this protein. When the magnetic immuno-nanoplatform is added into infected serum, it conjugates with the NS1 protein and can then be easily separated using an external magnetic field; then, the recovered immunoconjugate is transferred into a well containing a second immobilized NS1-antibody to form an ELISA-type system. When the NS1 protein is present, a color change to blue is induced by reaction with the Perls reagent, which is consistent with the formation of a SPION-antibody-NS1 antigen-antibody conjugate that confirms infection. No false positives were found when NS1 was not present or a different antibody and the NS1 protein were added into the system. The experimental findings could be extrapolated and scaled up to lead to future developments of simple, quick, and inexpensive, in situ biomolecular diagnostic tests for emergent viral infections.
AB - In this work, as a proof of principle, the design and performance evaluation of a simple, cheap and efficient colorimetric test for the detection of the NS1 protein of dengue virus, assisted by an immunoconjugate of magnetite (Fe3O4) nanoparticles coupled to anti-NS1 antibodies is reported. A monoclonal antibody against the NS1 antigen was covalently immobilized on the surface of superparamagnetic iron oxide nanoparticles (SPIONs ∼ 20 nm) and used for the immunodetection of this protein. When the magnetic immuno-nanoplatform is added into infected serum, it conjugates with the NS1 protein and can then be easily separated using an external magnetic field; then, the recovered immunoconjugate is transferred into a well containing a second immobilized NS1-antibody to form an ELISA-type system. When the NS1 protein is present, a color change to blue is induced by reaction with the Perls reagent, which is consistent with the formation of a SPION-antibody-NS1 antigen-antibody conjugate that confirms infection. No false positives were found when NS1 was not present or a different antibody and the NS1 protein were added into the system. The experimental findings could be extrapolated and scaled up to lead to future developments of simple, quick, and inexpensive, in situ biomolecular diagnostic tests for emergent viral infections.
UR - http://www.scopus.com/inward/record.url?scp=85088464694&partnerID=8YFLogxK
U2 - 10.1039/d0na00251h
DO - 10.1039/d0na00251h
M3 - Article
AN - SCOPUS:85088464694
SN - 2516-0230
VL - 2
SP - 3017
EP - 3026
JO - Nanoscale Advances
JF - Nanoscale Advances
IS - 7
ER -