A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material

B. Budowle; A. M. Gambel

doi:10.1520/jfs12482j

A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material

B. Budowle, A. M. Gambel

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Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).

Original language	English
Pages (from-to)	738-743
Number of pages	6
Journal	Journal of Forensic Sciences
Volume	33
Issue number	3
DOIs	https://doi.org/10.1520/jfs12482j
State	Published - 1988

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abstract = "An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).",

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year = "1988",

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AB - An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).

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DO - 10.1520/jfs12482j

M3 - Article

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A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material

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