A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material

B. Budowle, A. M. Gambel

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).

Original languageEnglish
Pages (from-to)738-743
Number of pages6
JournalJournal of Forensic Sciences
Volume33
Issue number3
DOIs
StatePublished - 1988

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