A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Sangram Limbaji Raut, Joseph Kimball, Rafal Fudala, Hung Doan, Badri Maliwal, Nirupama Sabnis, Andras G. Lacko, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.

Original languageEnglish
Pages (from-to)27037-27042
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume16
Issue number48
DOIs
StatePublished - 19 Nov 2014

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rotors
Rotors
Fluorescence
Viscosity
viscosity
membranes
Membranes
fluorescence
sensors
Sensors
Coloring Agents
dyes
life (durability)
cytoplasm
cholesterol
Viscosity measurement
cells
lipids
Microscopic examination
Cholesterol

Cite this

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abstract = "Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.",
author = "Raut, {Sangram Limbaji} and Joseph Kimball and Rafal Fudala and Hung Doan and Badri Maliwal and Nirupama Sabnis and Lacko, {Andras G.} and Ignacy Gryczynski and Dzyuba, {Sergei V.} and Zygmunt Gryczynski",
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A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments. / Raut, Sangram Limbaji; Kimball, Joseph; Fudala, Rafal; Doan, Hung; Maliwal, Badri; Sabnis, Nirupama; Lacko, Andras G.; Gryczynski, Ignacy; Dzyuba, Sergei V.; Gryczynski, Zygmunt.

In: Physical Chemistry Chemical Physics, Vol. 16, No. 48, 19.11.2014, p. 27037-27042.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

AU - Raut, Sangram Limbaji

AU - Kimball, Joseph

AU - Fudala, Rafal

AU - Doan, Hung

AU - Maliwal, Badri

AU - Sabnis, Nirupama

AU - Lacko, Andras G.

AU - Gryczynski, Ignacy

AU - Dzyuba, Sergei V.

AU - Gryczynski, Zygmunt

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