A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Sangram Raut; Joseph Kimball; Rafal Fudala; Hung Doan; Badri Maliwal; Nirupama Sabnis; Andras Lacko; Ignacy Gryczynski; Sergei V. Dzyuba; Zygmunt Gryczynski

doi:10.1039/c4cp04260c

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Sangram Raut, Joseph Kimball, Rafal Fudala, Hung Doan, Badri Maliwal, Nirupama Sabnis, Andras Lacko, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.

Original language	English
Pages (from-to)	27037-27042
Number of pages	6
Journal	Physical Chemistry Chemical Physics
Volume	16
Issue number	48
DOIs	https://doi.org/10.1039/c4cp04260c
State	Published - 19 Nov 2014

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abstract = "Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.",

author = "Sangram Raut and Joseph Kimball and Rafal Fudala and Hung Doan and Badri Maliwal and Nirupama Sabnis and Andras Lacko and Ignacy Gryczynski and Dzyuba, {Sergei V.} and Zygmunt Gryczynski",

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doi = "10.1039/c4cp04260c",

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AU - Raut, Sangram

AU - Kimball, Joseph

AU - Fudala, Rafal

AU - Doan, Hung

AU - Maliwal, Badri

AU - Sabnis, Nirupama

AU - Lacko, Andras

AU - Gryczynski, Ignacy

AU - Dzyuba, Sergei V.

AU - Gryczynski, Zygmunt

PY - 2014/11/19

Y1 - 2014/11/19

N2 - Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.

AB - Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.

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DO - 10.1039/c4cp04260c

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SN - 1463-9076

VL - 16

SP - 27037

EP - 27042

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 48

ER -

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

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