A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Sangram Raut, Joseph Kimball, Rafal Fudala, Hung Doan, Badri Maliwal, Nirupama Sabnis, Andras Lacko, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski

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Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.

Original languageEnglish
Pages (from-to)27037-27042
Number of pages6
JournalPhysical Chemistry Chemical Physics
Issue number48
StatePublished - 19 Nov 2014


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