TY - JOUR
T1 - A high resolution, rapid procedure for alpha 1‐antitrypsin phenotyping
AU - Budowle, Bruce
AU - Murch, Randall S.
PY - 1985
Y1 - 1985
N2 - An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for typing alpha 1‐antritrypsin. This method, which uses Pharmalyte pH 4.2–4.9 and a 9.5 cm or 7.0 cm inter‐electrode wick distance, clearly resolves the M1, M2, M3, S, Z and a number of rare variant allelic products. In addition, a rapid screening method for the detection of the Z, the most deficient variant, using a gel with an electrode wick distance of 5.0 cm, is also efficaceous in the detection of this and other rare allelic products.
AB - An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for typing alpha 1‐antritrypsin. This method, which uses Pharmalyte pH 4.2–4.9 and a 9.5 cm or 7.0 cm inter‐electrode wick distance, clearly resolves the M1, M2, M3, S, Z and a number of rare variant allelic products. In addition, a rapid screening method for the detection of the Z, the most deficient variant, using a gel with an electrode wick distance of 5.0 cm, is also efficaceous in the detection of this and other rare allelic products.
UR - http://www.scopus.com/inward/record.url?scp=0022253183&partnerID=8YFLogxK
U2 - 10.1002/elps.1150061012
DO - 10.1002/elps.1150061012
M3 - Article
AN - SCOPUS:0022253183
SN - 0173-0835
VL - 6
SP - 523
EP - 525
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 10
ER -