A genomic level analysis of alcohol producing pathways of Zymomonas mobilis

Amalendu Prakash Ranjan, C. S. Prasad, G. P. Agarwal, S. Ramachandran

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The need of alternative energy source has intensified the efforts to produce bio-ethanol and study the efficiency of its industrial processes using microorganisms. Our work intends to study and analyze the alcohol producing pathways of bacteria, signifying the importance of regulatory sequence, on genomic level. Zymomonas mobilis, considered as a potent future organism for alcohol production, was chosen for this study. The pathway was analysed on the basis of promoter regulation, a key factor for synthesis of enzymes. It was found that the second TA base pair could be central to the binding of TATA box-binding protein (TBP) to TATA box. The length of the TATA box, based on analysis of bending stiffness energy ratio of left sequence to right sequence of central base pair, is proposed to be 10. A mathematical model for the prediction of promoter strength was developed based on bending stiffness energy of flanking sequence of TATA box. The relative promoter strengths of all the genes, of the enzymes that take part in alcohol production, were predicted using this model. Results were analyzed and the predictions of the model for the bacteria, Zymomonas mobilis, were supported by available experimental literature.

Original languageEnglish
Title of host publicationICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering
Pages471-474
Number of pages4
DOIs
StatePublished - 1 Dec 2006
EventICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering - Singapore, Singapore
Duration: 11 Dec 200614 Dec 2006

Other

OtherICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering
CountrySingapore
CitySingapore
Period11/12/0614/12/06

Keywords

  • Bending stiffness energy
  • Relative strength
  • TATA box

Fingerprint Dive into the research topics of 'A genomic level analysis of alcohol producing pathways of Zymomonas mobilis'. Together they form a unique fingerprint.

  • Cite this

    Ranjan, A. P., Prasad, C. S., Agarwal, G. P., & Ramachandran, S. (2006). A genomic level analysis of alcohol producing pathways of Zymomonas mobilis. In ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering (pp. 471-474). [4155947] https://doi.org/10.1109/ICBPE.2006.348638