A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats

Hijab Ahmed, Johanna L. Hannan, John W. Apolzan, Oluwatobiloba Osikoya, Spencer C. Cushen, Steven Anthony Romero, Styliani Goulopoulou

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats. In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance. In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction. When the free-choice diet continued throughout pregnancy, rats remained hyperphagic, hyperleptinemic, and obese. Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats. Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats. These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents. Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.

Original languageEnglish
Pages (from-to)R472-R485
JournalAmerican journal of physiology. Regulatory, integrative and comparative physiology
Volume316
Issue number5
DOIs
StatePublished - 1 May 2019

Fingerprint

Hyperphagia
High Fat Diet
Sucrose
Obesity
Mothers
Diet
Energy Intake
Weights and Measures
Pregnancy
Pancreas
Arterial Pressure
Uterine Artery
Glucose Intolerance
Adiposity
Leptin
Body Composition
Sprague Dawley Rats
Rodentia
Triglycerides
Fetus

Keywords

  • diet-induced obesity
  • energy intake
  • female
  • uterine artery
  • vascular function

Cite this

@article{c45dc8edcac64b628aac11143b58a737,
title = "A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats",
abstract = "The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats. In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance. In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction. When the free-choice diet continued throughout pregnancy, rats remained hyperphagic, hyperleptinemic, and obese. Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats. Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats. These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents. Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.",
keywords = "diet-induced obesity, energy intake, female, uterine artery, vascular function",
author = "Hijab Ahmed and Hannan, {Johanna L.} and Apolzan, {John W.} and Oluwatobiloba Osikoya and Cushen, {Spencer C.} and Romero, {Steven Anthony} and Styliani Goulopoulou",
year = "2019",
month = "5",
day = "1",
doi = "10.1152/ajpregu.00391.2018",
language = "English",
volume = "316",
pages = "R472--R485",
journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "5",

}

A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats. / Ahmed, Hijab; Hannan, Johanna L.; Apolzan, John W.; Osikoya, Oluwatobiloba; Cushen, Spencer C.; Romero, Steven Anthony; Goulopoulou, Styliani.

In: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 316, No. 5, 01.05.2019, p. R472-R485.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats

AU - Ahmed, Hijab

AU - Hannan, Johanna L.

AU - Apolzan, John W.

AU - Osikoya, Oluwatobiloba

AU - Cushen, Spencer C.

AU - Romero, Steven Anthony

AU - Goulopoulou, Styliani

PY - 2019/5/1

Y1 - 2019/5/1

N2 - The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats. In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance. In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction. When the free-choice diet continued throughout pregnancy, rats remained hyperphagic, hyperleptinemic, and obese. Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats. Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats. These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents. Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.

AB - The main objective of these studies was to characterize metabolic, body composition, and cardiovascular responses to a free-choice high-fat, high-sucrose diet in female cycling and pregnant rats. In the nonpregnant state, female Sprague-Dawley rats offered a 3-wk free-choice high-fat, high-sucrose diet had greater energy intake, adiposity, serum leptin, and triglyceride concentrations compared with rats fed with standard chow and developed glucose intolerance. In addition, choice-diet-fed rats had larger cardiac ventricular weights, smaller kidney and pancreas weights, and higher blood pressure than chow-fed rats, but they did not exhibit resistance artery endothelial dysfunction. When the free-choice diet continued throughout pregnancy, rats remained hyperphagic, hyperleptinemic, and obese. Choice pregnant rats exhibited uterine artery endothelial dysfunction and had smaller fetuses compared with chow pregnant rats. Pregnancy normalized mean arterial blood pressure and pancreas weights in choice rats. These studies are the first to provide a comprehensive evaluation of free-choice high-fat, high-sucrose diet on metabolic and cardiovascular functions in female rats, extending the previous studies in males to female cycling and pregnant rodents. Free-choice diet may provide a new model of preconceptual maternal obesity to study the role of increased energy intake, individual food components, and preexisting maternal obesity on maternal and offspring physiological responses during pregnancy and after birth.

KW - diet-induced obesity

KW - energy intake

KW - female

KW - uterine artery

KW - vascular function

UR - http://www.scopus.com/inward/record.url?scp=85065050649&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00391.2018

DO - 10.1152/ajpregu.00391.2018

M3 - Article

VL - 316

SP - R472-R485

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 5

ER -