A comparison and integration of MiSeq and MinION platforms for sequencing single source and mixed mitochondrial genomes

Michael R. Lindberg, Sarah E. Schmedes, F. Curtis Hewitt, Jamie L. Haas, Krista L. Ternus, Dana R. Kadavy, Bruce Budowle

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Single source and multiple donor (mixed) samples of human mitochondrial DNA were analyzed and compared using the MinION and the MiSeq platforms. A generalized variant detection strategy was employed to provide a cursory framework for evaluating the reliability and accuracy of mitochondrial sequences produced by the MinION. The feasibility of longread phasing was investigated to establish its efficacy in quantitatively distinguishing and deconvolving individuals in a mixture. Finally, a proof-of-concept was demonstrated by integrating both platforms in a hybrid assembly that leverages solely mixture data to accurately reconstruct full mitochondrial genomes.

Original languageEnglish
Article numbere0167600
JournalPLoS ONE
Volume11
Issue number12
DOIs
StatePublished - Dec 2016

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