A comparative study of phytoestrogen action in mitigating apoptosis induced by oxidative stress

Amber J. Ondricek, Abhishek K. Kashyap, Sanjay I. Thamake, Jamboor K. Vishwanatha

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The phytoestrogens kaempferol, genistein and genistin were characterized using in vitro assays utilizing iodoacetic acid (IAA)-induced oxidative stress and mitochondrial dysfunction. RGC-5 cells were treated with different concentrations of IAA, and phytoestrogens were administered along with IAA. IAA is cytotoxic to RGC-5 cells and induces the generation of reactive oxygen species (ROS) in vitro. Genistein rescued RGC-5 cells in the presence of IAA, however, it also increased caspase activation and did not inhibit the generation of ROS. Genistein increased phosphorylation of ribosomal s6 kinase (p90RSK), reduced phosphorylation of the ribosomal S6 protein, and had no effect on phosphorylation of protein kinase B (AKT). Kaempferol and genistin rescued RGC-5 cells from IAA-induced cell death, as well as reduced caspase activation and ROS generation. Kaempferol increased phosphorylation of AKT and MAP kinase (p44/42). Genistin reduced phosphorylation of p42 and p90RSK. Although these phytoestrogens are flavonoids and similar in structure, they exhibit different effects on cell signaling.

Original languageEnglish
Pages (from-to)765-775
Number of pages11
JournalIn Vivo
Volume26
Issue number5
StatePublished - 1 Sep 2012

Fingerprint

Iodoacetic Acid
Phytoestrogens
Oxidative stress
Phosphorylation
Oxidative Stress
Apoptosis
Genistein
Reactive Oxygen Species
Caspases
Chemical activation
Ribosomal Protein S6
Ribosomal Protein S6 Kinases
Cell signaling
Proto-Oncogene Proteins c-akt
Cell death
Flavonoids
Assays
Cell Death
Phosphotransferases
kaempferol

Keywords

  • Antioxidants
  • Glaucoma
  • Neurodegenerative disease
  • Neuroprotection
  • Oxidative stress
  • Phytoestrogens
  • RGC-5 cells

Cite this

Ondricek, Amber J. ; Kashyap, Abhishek K. ; Thamake, Sanjay I. ; Vishwanatha, Jamboor K. / A comparative study of phytoestrogen action in mitigating apoptosis induced by oxidative stress. In: In Vivo. 2012 ; Vol. 26, No. 5. pp. 765-775.
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A comparative study of phytoestrogen action in mitigating apoptosis induced by oxidative stress. / Ondricek, Amber J.; Kashyap, Abhishek K.; Thamake, Sanjay I.; Vishwanatha, Jamboor K.

In: In Vivo, Vol. 26, No. 5, 01.09.2012, p. 765-775.

Research output: Contribution to journalArticle

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