TY - JOUR
T1 - A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance.
AU - Kimple, Adam J.
AU - Muller, Robin E.
AU - Siderovski, David P.
AU - Willard, Francis S.
PY - 2010
Y1 - 2010
N2 - Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.
AB - Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.
UR - http://www.scopus.com/inward/record.url?scp=77953275236&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-670-2_5
DO - 10.1007/978-1-60761-670-2_5
M3 - Article
C2 - 20217615
AN - SCOPUS:77953275236
SN - 1064-3745
VL - 627
SP - 91
EP - 100
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -