A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance.

Adam J. Kimple, Robin E. Muller, David P. Siderovski, Francis S. Willard

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.

Original languageEnglish
Pages (from-to)91-100
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume627
DOIs
StatePublished - 2010

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