We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.
|Number of pages||13|
|Journal||Genes and Development|
|State||Published - 15 Dec 1997|
- MRNA cap
- RNA 7-methyltransferase
- RNA Pol II
- RNA guanylyltransferase