Abstract
We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.
Original language | English |
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Pages (from-to) | 3306-3318 |
Number of pages | 13 |
Journal | Genes and Development |
Volume | 11 |
Issue number | 24 |
DOIs | |
State | Published - 15 Dec 1997 |
Keywords
- CTD
- MRNA cap
- RNA 7-methyltransferase
- RNA Pol II
- RNA guanylyltransferase