5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II

Susan McCracken; Nova Fong; Emanuel Rosonina; Krassimir Yankulov; Greg Brothers; David Siderovski; Andrew Hessel; Stephen Foster; Stewart Shuman; David L. Bentley

doi:10.1101/gad.11.24.3306

5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II

Susan McCracken, Nova Fong, Emanuel Rosonina, Krassimir Yankulov, Greg Brothers, David Siderovski, Andrew Hessel, Stephen Foster, Stewart Shuman, David L. Bentley

Research output: Contribution to journal › Article › peer-review

435 Scopus citations

Abstract

We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.

Original language	English
Pages (from-to)	3306-3318
Number of pages	13
Journal	Genes and Development
Volume	11
Issue number	24
DOIs	https://doi.org/10.1101/gad.11.24.3306
State	Published - 15 Dec 1997

Keywords

CTD
MRNA cap
RNA 7-methyltransferase
RNA Pol II
RNA guanylyltransferase

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title = "5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II",

abstract = "We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.",

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author = "Susan McCracken and Nova Fong and Emanuel Rosonina and Krassimir Yankulov and Greg Brothers and David Siderovski and Andrew Hessel and Stephen Foster and Stewart Shuman and Bentley, {David L.}",

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T1 - 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II

AU - McCracken, Susan

AU - Fong, Nova

AU - Rosonina, Emanuel

AU - Yankulov, Krassimir

AU - Brothers, Greg

AU - Siderovski, David

AU - Hessel, Andrew

AU - Foster, Stephen

AU - Shuman, Stewart

AU - Bentley, David L.

PY - 1997/12/15

Y1 - 1997/12/15

N2 - We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.

AB - We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.

KW - CTD

KW - MRNA cap

KW - RNA 7-methyltransferase

KW - RNA Pol II

KW - RNA guanylyltransferase

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SN - 0890-9369

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SP - 3306

EP - 3318

JO - Genes and Development

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ER -

5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II

Abstract

Keywords

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