2B4(CD244)-Mediated Activation of NK Cells Reduces Metastases of B16F10 Melanoma in Mice

Lori A. Johnson, Swapnil V. Vaidya, Ronald H. Goldfarb, Porunelloor A. Mathew

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Natural killer (NK) cells are a third population of lymphocytes that can kill certain tumor cells. This killing is regulated by signals received through activating and inhibitory receptors. 2B4 (CD244), a member of the CD2 subset of Immunoglobulin superfamily, was identified as an activating receptor on NK cells. Interaction of 2B4 with its ligand CD48 or anti-2B4 mAb stimulates NK cell cytolytic function as well as production of INF-γ. Materials and Methods: A murine tumor model was used to study the in vivo role of 2B4. 2B4 and CD48 were activated in vivo by injecting anti-2B4 and anti-CD48 monoclonal antibodies. Results: Activation of 2B4 or CD48 resulted in a five-fold reduction in tumor metastasis. IFN-γ knockout mice had a two-fold increase in metastasis as compared to wild-type after 2B4 activation. Conclusion: Activation of 2B4 and CD48 reduces metastasis of B16F10 melanoma cells and this anti-tumor effect involves both cytolytic function and cytokine production.

Original languageEnglish
Pages (from-to)3651-3655
Number of pages5
JournalAnticancer Research
Volume23
Issue number5 A
StatePublished - 1 Sep 2003

Fingerprint

Natural Killer Cells
Melanoma
Neoplasm Metastasis
Neoplasms
Natural Killer Cell Receptors
Knockout Mice
Immunoglobulins
Monoclonal Antibodies
Lymphocytes
Cytokines
Ligands
Population

Keywords

  • 2B4
  • Immunotherapy
  • Melanoma
  • NK cells

Cite this

Johnson, Lori A. ; Vaidya, Swapnil V. ; Goldfarb, Ronald H. ; Mathew, Porunelloor A. / 2B4(CD244)-Mediated Activation of NK Cells Reduces Metastases of B16F10 Melanoma in Mice. In: Anticancer Research. 2003 ; Vol. 23, No. 5 A. pp. 3651-3655.
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abstract = "Background: Natural killer (NK) cells are a third population of lymphocytes that can kill certain tumor cells. This killing is regulated by signals received through activating and inhibitory receptors. 2B4 (CD244), a member of the CD2 subset of Immunoglobulin superfamily, was identified as an activating receptor on NK cells. Interaction of 2B4 with its ligand CD48 or anti-2B4 mAb stimulates NK cell cytolytic function as well as production of INF-γ. Materials and Methods: A murine tumor model was used to study the in vivo role of 2B4. 2B4 and CD48 were activated in vivo by injecting anti-2B4 and anti-CD48 monoclonal antibodies. Results: Activation of 2B4 or CD48 resulted in a five-fold reduction in tumor metastasis. IFN-γ knockout mice had a two-fold increase in metastasis as compared to wild-type after 2B4 activation. Conclusion: Activation of 2B4 and CD48 reduces metastasis of B16F10 melanoma cells and this anti-tumor effect involves both cytolytic function and cytokine production.",
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Johnson, LA, Vaidya, SV, Goldfarb, RH & Mathew, PA 2003, '2B4(CD244)-Mediated Activation of NK Cells Reduces Metastases of B16F10 Melanoma in Mice', Anticancer Research, vol. 23, no. 5 A, pp. 3651-3655.

2B4(CD244)-Mediated Activation of NK Cells Reduces Metastases of B16F10 Melanoma in Mice. / Johnson, Lori A.; Vaidya, Swapnil V.; Goldfarb, Ronald H.; Mathew, Porunelloor A.

In: Anticancer Research, Vol. 23, No. 5 A, 01.09.2003, p. 3651-3655.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 2B4(CD244)-Mediated Activation of NK Cells Reduces Metastases of B16F10 Melanoma in Mice

AU - Johnson, Lori A.

AU - Vaidya, Swapnil V.

AU - Goldfarb, Ronald H.

AU - Mathew, Porunelloor A.

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Background: Natural killer (NK) cells are a third population of lymphocytes that can kill certain tumor cells. This killing is regulated by signals received through activating and inhibitory receptors. 2B4 (CD244), a member of the CD2 subset of Immunoglobulin superfamily, was identified as an activating receptor on NK cells. Interaction of 2B4 with its ligand CD48 or anti-2B4 mAb stimulates NK cell cytolytic function as well as production of INF-γ. Materials and Methods: A murine tumor model was used to study the in vivo role of 2B4. 2B4 and CD48 were activated in vivo by injecting anti-2B4 and anti-CD48 monoclonal antibodies. Results: Activation of 2B4 or CD48 resulted in a five-fold reduction in tumor metastasis. IFN-γ knockout mice had a two-fold increase in metastasis as compared to wild-type after 2B4 activation. Conclusion: Activation of 2B4 and CD48 reduces metastasis of B16F10 melanoma cells and this anti-tumor effect involves both cytolytic function and cytokine production.

AB - Background: Natural killer (NK) cells are a third population of lymphocytes that can kill certain tumor cells. This killing is regulated by signals received through activating and inhibitory receptors. 2B4 (CD244), a member of the CD2 subset of Immunoglobulin superfamily, was identified as an activating receptor on NK cells. Interaction of 2B4 with its ligand CD48 or anti-2B4 mAb stimulates NK cell cytolytic function as well as production of INF-γ. Materials and Methods: A murine tumor model was used to study the in vivo role of 2B4. 2B4 and CD48 were activated in vivo by injecting anti-2B4 and anti-CD48 monoclonal antibodies. Results: Activation of 2B4 or CD48 resulted in a five-fold reduction in tumor metastasis. IFN-γ knockout mice had a two-fold increase in metastasis as compared to wild-type after 2B4 activation. Conclusion: Activation of 2B4 and CD48 reduces metastasis of B16F10 melanoma cells and this anti-tumor effect involves both cytolytic function and cytokine production.

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