2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48

Marion H. Brown; Kent Boles; P. Anton Van Der Merwe; Vinay Kumar; Porunelloor A. Mathew; A. Neil Barclay

doi:10.1084/jem.188.11.2083

2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48

Marion H. Brown, Kent Boles, P. Anton Van Der Merwe, Vinay Kumar, Porunelloor A. Mathew, A. Neil Barclay

Research output: Contribution to journal › Article

337 Scopus citations

Abstract

2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin- coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (K(d)≃ 16 μM at 37°C) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (K(d) ≃ 8 μM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.

Original language	English
Pages (from-to)	2083-2090
Number of pages	8
Journal	Journal of Experimental Medicine
Volume	188
Issue number	11
DOIs	https://doi.org/10.1084/jem.188.11.2083
State	Published - 7 Dec 1998

Keywords

2B4
CD48
Ligand
Natural killer cell
T cell

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Brown, M. H., Boles, K., Van Der Merwe, P. A., Kumar, V., Mathew, P. A., & Barclay, A. N. (1998). 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. Journal of Experimental Medicine, 188(11), 2083-2090. https://doi.org/10.1084/jem.188.11.2083

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AU - Brown, Marion H.

AU - Boles, Kent

AU - Van Der Merwe, P. Anton

AU - Kumar, Vinay

AU - Mathew, Porunelloor A.

AU - Barclay, A. Neil

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AB - 2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin- coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (K(d)≃ 16 μM at 37°C) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (K(d) ≃ 8 μM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.

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