Abstract
2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin- coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (K(d)≃ 16 μM at 37°C) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (K(d) ≃ 8 μM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.
Original language | English |
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Pages (from-to) | 2083-2090 |
Number of pages | 8 |
Journal | Journal of Experimental Medicine |
Volume | 188 |
Issue number | 11 |
DOIs | |
State | Published - 7 Dec 1998 |
Keywords
- 2B4
- CD48
- Ligand
- Natural killer cell
- T cell