Srikantha Thyagarajan

2017

Protocol for identifying natural agents that selectively affect adhesion, thickness, architecture, cellular phenotypes, extracellular matrix, and human white blood cell impenetrability of candida albicans biofilms

Park, Y. N., Srikantha, T., Daniels, K. J., Jacob, M. R., Agarwal, A. K., Li, X. C. & Solla, D. R., Nov 2017, In : Antimicrobial agents and chemotherapy. 61, 11, e01319-17.

Research output: Contribution to journal › Article

Biofilms

Candida albicans

Extracellular Matrix

Leukocytes

Phenotype

2016

Binding sites in the EFG1 promoter for transcription factors in a proposed regulatory network: A functional analysis in the white and opaque phases of Candida albicans

Pujol, C., Srikantha, T., Park, Y. N., Daniels, K. J. & Soll, D. R., 1 Jan 2016, In : G3: Genes, Genomes, Genetics. 6, 6, p. 1725-1737 13 p.

Research output: Contribution to journal › Article

Candida albicans

Transcription Factors

Binding Sites

Renilla Luciferases

Phenotype

2015

10 Citations (Scopus)

Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans

Daniels, K. J., Srikantha, T., Pujol, C., Park, Y. N. & Soll, D. R., 1 Jan 2015, In : Eukaryotic Cell. 14, 3, p. 228-240 13 p.

Research output: Contribution to journal › Article

Biofilms

Candida albicans

Silicone Elastomers

Mitogen-Activated Protein Kinases

Hyphae

2013

31 Citations (Scopus)

Candida albicans forms a specialized "sexual" as well as "pathogenic" biofilm

Park, Y. N., Daniels, K. J., Pujol, C., Srikantha, T. & Soll, D. R., 1 Aug 2013, In : Eukaryotic Cell. 12, 8, p. 1120-1131 12 p.

Research output: Contribution to journal › Article

Biofilms

Candida albicans

Fluconazole

Mitogen-Activated Protein Kinases

Cyclic AMP

28 Citations (Scopus)

Identification of genes upregulated by the transcription factor bcr1 that are involved in impermeability, impenetrability, and drug resistance of Candida albicans a/α biofilms

Thyagarajan, S., Daniels, K. J., Pujol, C., Kim, E. & Soll, D. R., 1 Jun 2013, In : Eukaryotic Cell. 12, 6, p. 875-888 14 p.

Research output: Contribution to journal › Article

Biofilms

Candida albicans

Drug Resistance

Transcription Factors

Genes

28 Citations (Scopus)

Impact of environmental conditions on the form and function of Candida albicans biofilms

Daniels, K. J., Park, Y. N., Thyagarajan, S., Pujol, C. & Soll, D. R., 1 Oct 2013, In : Eukaryotic Cell. 12, 10, p. 1389-1402 14 p.

Research output: Contribution to journal › Article

Biofilms

Candida albicans

Spiders

Air

Fluconazole

2012

21 Citations (Scopus)

Nonsex genes in the mating type locus of Candida albicans play roles in a/α biofilm formation, including impermeability and fluconazole resistance

Srikantha, T., Daniels, K. J., Pujol, C., Sahni, N., Yi, S. & Soll, D. R., 1 Jan 2012, In : PLoS Pathogens. 8, 1, e1002476.

Research output: Contribution to journal › Article

Fluconazole

Biofilms

Candida albicans

Genes

Co-Repressor Proteins

8 Citations (Scopus)

The "finger," a Unique multicellular morphology of Candida albicans induced by CO₂ and dependent upon the ras1-cyclic AMP pathway

Daniels, K. J., Pujol, C., Srikantha, T. & Soll, D. R., 1 Oct 2012, In : Eukaryotic Cell. 11, 10, p. 1257-1267 11 p.

Research output: Contribution to journal › Article

Candida albicans

Cyclic AMP

Fingers

Yeasts

Agar

2011

65 Citations (Scopus)

Alternative mating type configurations (a/α versus a/a or α/α) of candida albicans result in alternative biofilms regulated by different pathways

Yi, S., Sahni, N., Daniels, K. J., Lu, K. L., Srikantha, T., Huang, G., Garnaas, A. M. & Soll, D. R., 1 Aug 2011, In : PLoS Biology. 9, 8, e1001117.

Research output: Contribution to journal › Article

Candida

Biofilms

Candida albicans

biofilm

Signal transduction

16 Citations (Scopus)

Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Yang, M., Brand, A., Srikantha, T., Daniels, K. J., Soll, D. R. & Gow, N. A. R., 1 Mar 2011, In : Eukaryotic Cell. 10, 3, p. 435-444 10 p.

Research output: Contribution to journal › Article

Green Fluorescent Proteins

Candida albicans

Pheromones

Calcium

Membranes

19 Citations (Scopus)

Self-induction of a/a or α/α biofilms in Candida albicans is a pheromone-based paracrine system requiring switching

Yi, S., Sahni, N., Daniels, K. J., Lu, K. L., Huang, G., Srikantha, T. & Soll, D. R., 1 Jun 2011, In : Eukaryotic Cell. 10, 6, p. 753-760 8 p.

Research output: Contribution to journal › Article

Pheromones

Biofilms

Candida albicans

Genes

Pheromone Receptors

41 Citations (Scopus)

Target specificity of the Candida albicans Efg1 regulator

Lassak, T., Schneider, E., Bussmann, M., Kurtz, D., Manak, J. R., Thyagarajan, S., Soll, D. R. & Ernst, J. F., 1 Nov 2011, In : Molecular Microbiology. 82, 3, p. 602-618 17 p.

Research output: Contribution to journal › Article

Candida albicans

Yeasts

Growth

Down-Regulation

Genome

16 Citations (Scopus)

Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development

Yi, S., Sahni, N., Daniels, K. J., Lu, K. L., Huang, G., Garnaas, A. M., Pujol, C., Srikantha, T. & Soll, D. R., 1 Jan 2011, In : mBio. 2, 1

Research output: Contribution to journal › Article

Biofilms

Signal Transduction

Pheromones

Mitogen-Activated Protein Kinases

Phenotype

2010

138 Citations (Scopus)

N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans

Huang, G., Yi, S., Sahni, N., Daniels, K. J., Thyagarajan, S. & Soll, D. R., 1 Mar 2010, In : PLoS Pathogens. 6, 3, e1000806.

Research output: Contribution to journal › Article

Acetylglucosamine

Candida albicans

Gastrointestinal Tract

Temperature

Monosaccharides

57 Citations (Scopus)

Tec1 mediates the pheromone response of the white phenotype of Candida albicans: Insights into the evolution of new signal transduction pathways

Sahni, N., Yi, S., Daniels, K. J., Huang, G., Srikantha, T. & Soll, D. R., 1 May 2010, In : PLoS Biology. 8, 5

Research output: Contribution to journal › Article

Signal transduction

Candida

Pheromones

Candida albicans

pheromones

2009

28 Citations (Scopus)

A Candida albicans-specific region of the α-pheromone receptor plays a selective role in the white cell pheromone response

Yi, S., Sahni, N., Pujol, C., Daniels, K. J., Thyagarajan, S., Ma, N. & Soll, D. R., 1 Feb 2009, In : Molecular Microbiology. 71, 4, p. 925-947 23 p.

Research output: Contribution to journal › Article

Pheromone Receptors

Pheromones

Candida albicans

Biofilms

Mitogen-Activated Protein Kinases

112 Citations (Scopus)

CO2 Regulates White-to-Opaque Switching in Candida albicans

Huang, G., Thyagarajan, S., Sahni, N., Yi, S. & Soll, D. R., 24 Feb 2009, In : Current Biology. 19, 4, p. 330-334 5 p.

Research output: Contribution to journal › Article

Candida

Candida albicans

carbon dioxide

Switches

Phenotype

592 Citations (Scopus)

Evolution of pathogenicity and sexual reproduction in eight Candida genomes

Butler, G., Rasmussen, M. D., Lin, M. F., Santos, M. A. S., Sakthikumar, S., Munro, C. A., Rheinbay, E., Grabherr, M., Forche, A., Reedy, J. L., Agrafioti, I., Arnaud, M. B., Bates, S., Brown, A. J. P., Brunke, S., Costanzo, M. C., Fitzpatrick, D. A., De Groot, P. W. J., Harris, D., Hoyer, L. L. & 31 others, Hube, B., Klis, F. M., Kodira, C., Lennard, N., Logue, M. E., Martin, R., Neiman, A. M., Nikolaou, E., Quail, M. A., Quinn, J., Santos, M. C., Schmitzberger, F. F., Sherlock, G., Shah, P., Silverstein, K. A. T., Skrzypek, M. S., Soll, D., Staggs, R., Stansfield, I., Stumpf, M. P. H., Sudbery, P. E., Thyagarajan, S., Zeng, Q., Berman, J., Berriman, M., Heitman, J., Gow, N. A. R., Lorenz, M. C., Birren, B. W., Kellis, M. & Cuomo, C. A., 4 Jun 2009, In : Nature. 459, 7247, p. 657-662 6 p.

Research output: Contribution to journal › Article

Candida

Reproduction

Virulence

Genome

Genes

46 Citations (Scopus)

Genes selectively up-regulated by pheromone in white cells are involved in biofilm formation in Candida albicans.

Sahni, N., Yi, S., Daniels, K. J., Thyagarajan, S., Pujol, C. & Soll, D. R., 1 Jan 2009, In : PLoS Pathogens. 5, 10

Research output: Contribution to journal › Article

Pheromones

Biofilms

Candida albicans

Genes

Virulence Factors

2008

17 Citations (Scopus)

Dark brown is the more virulent of the switch phenotypes of Candida glabrata

Srikantha, T., Daniels, K. J., Wu, W., Lockhart, S. R., Yi, S., Sahni, N., Ma, N. & Soll, D. R., 15 Dec 2008, In : Microbiology. 154, 11, p. 3309-3318 10 p.

Research output: Contribution to journal › Article

Candida glabrata

Phenotype

Spleen

Virulence

Liver

48 Citations (Scopus)

The same receptor, G protein, and mitogen-activated protein kinase pathway activate different downstream regulators in the alternative white and opaque pheromone responses of Candida albicans

Yi, S., Sahni, N., Daniels, K. J., Pujol, C., Srikantha, T. & Soll, D. R., 1 Mar 2008, In : Molecular Biology of the Cell. 19, 3, p. 957-970 14 p.

Research output: Contribution to journal › Article

Pheromones

Mitogen-Activated Protein Kinases

Candida albicans

GTP-Binding Proteins

Heterotrimeric GTP-Binding Proteins

2007

54 Citations (Scopus)

Heterozygosity of genes on the sex chromosome regulates Candida albicans virulence

Wu, W., Lockhart, S. R., Pujol, C., Thyagarajan, S. & Soll, D. R., 1 Jun 2007, In : Molecular Microbiology. 64, 6, p. 1587-1604 18 p.

Research output: Contribution to journal › Article

Chromosomes, Human, Pair 5

Sex Chromosomes

Candida albicans

Virulence

Uniparental Disomy

2006

119 Citations (Scopus)

Opaque cells signal white cells to form biofilms in Candida albicans

Daniels, K. J., Thyagarajan, S., Lockhart, S. R., Pujol, C. & Soll, D. R., 17 May 2006, In : EMBO Journal. 25, 10, p. 2240-2252 13 p.

Research output: Contribution to journal › Article

Candida

Pheromones

Biofilms

Candida albicans

Pheromone Receptors

36 Citations (Scopus)

The Shwachman-Bodian-Diamond syndrome gene encodes an RNA-binding protein that localizes to the pseudopod of Dictyostelium amoebae during chemotaxis

Wessels, D., Srikantha, T., Yi, S., Kuhl, S., Aravind, L. & Soll, D. R., 15 Jan 2006, In : Journal of Cell Science. 119, 2, p. 370-379 10 p.

Research output: Contribution to journal › Article

Pseudopodia

Amoeba

Dictyostelium

RNA-Binding Proteins

Chemotaxis

141 Citations (Scopus)

TOS9 regulates white-opaque switching in Candida albicans

Srikantha, T., Borneman, A. R., Daniels, K. J., Pujol, C., Wu, W., Seringhaus, M. R., Gerstein, M., Yi, S., Snyder, M. & Soll, D. R., 1 Oct 2006, In : Eukaryotic Cell. 5, 10, p. 1674-1687MP

Research output: Contribution to journal › Article

Candida albicans

Genes

Switch Genes

Co-Repressor Proteins

Chromatin Immunoprecipitation

2005

22 Citations (Scopus)

Phenotypic switching in Candida glabrata accompanied by changes in expression of genes with deduced functions in copper detoxification and stress

Srikantha, T., Zhao, R., Daniels, K., Radke, J. & Soll, D. R., 1 Aug 2005, In : Eukaryotic Cell. 4, 8, p. 1434-1445 12 p.

Research output: Contribution to journal › Article

Candida glabrata

Copper

Gene Expression

Phenotype

Genes

2004

74 Citations (Scopus)

The closely related species Candida albicans and Candida dubliniensis can mate

Pujol, C., Daniels, K. J., Lockhart, S. R., Srikantha, T., Radke, J. B., Geiger, J. & Soll, D. R., 1 Aug 2004, In : Eukaryotic Cell. 3, 4, p. 1015-1027 13 p.

Research output: Contribution to journal › Article

Candida albicans

Candida

Agar

Suspensions

Nuclear Fusion

2003

46 Citations (Scopus)

Phenotypic Switching and Mating Type Switching of Candida glabrata at Sites of Colonization

Brockert, P. J., Lachke, S. A., Srikantha, T., Pujol, C., Galask, R. & Soll, D. R., 1 Dec 2003, In : Infection and Immunity. 71, 12, p. 7109-7118 10 p.

Research output: Contribution to journal › Article

Candida glabrata

Vaginitis

Phenotype

Agar

Genetic Transcription

25 Citations (Scopus)

The regulation of EFG1 in white-opaque switching in Candida albicans involves overlapping promoters

Lachke, S. A., Thyagarajan, S. & Soll, D. R., 1 Apr 2003, In : Molecular Microbiology. 48, 2, p. 523-536 14 p.

Research output: Contribution to journal › Article

Candida albicans

Trans-Activators

Sequence Deletion

Electrophoretic Mobility Shift Assay

Luciferases

46 Citations (Scopus)

Three mating type-like loci in Candida glabrata

Thyagarajan, S., Lachke, S. A. & Soll, D. R., 1 Apr 2003, In : Eukaryotic Cell. 2, 2, p. 328-340 13 p.

Research output: Contribution to journal › Article

Candida glabrata

Saccharomyces cerevisiae

Genotype

Genes

Intergenic DNA

2001

58 Citations (Scopus)

A histone deacetylation inhibitor and mutant promote colony-type switching of the human pathogen Candida albicans

Klar, A. J. S., Thyagarajan, S. & Soll, D. R., 3 Jul 2001, In : Genetics. 158, 2, p. 919-924 6 p.

Research output: Contribution to journal › Article

trichostatin A

Candida albicans

Histones

Histone Deacetylase Inhibitors

Acetylation

89 Citations (Scopus)

The histone deacetylase genes HDA1 and RPD3 play distinct roles in regulation of high-frequency phenotypic switching in Candida albicans

Srikantha, T., Tsai, L., Daniels, K., Klar, A. J. S. & Soll, D. R., 25 Jul 2001, In : Journal of Bacteriology. 183, 15, p. 4614-4625 12 p.

Research output: Contribution to journal › Article

Histone Deacetylases

Candida albicans

Genes

Sequence Analysis

Gene Expression

2000

86 Citations (Scopus)

EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells

Srikantha, T., Tsai, L. K., Daniels, K. & Soll, D. R., 1 Mar 2000, In : Journal of Bacteriology. 182, 6, p. 1580-1591 12 p.

Research output: Contribution to journal › Article

Candida albicans

Phenotype

Complementary DNA

Null Lymphocytes

Messenger RNA

67 Citations (Scopus)

Phenotypic switching in Candida glabrata involves phase-specific regulation of the metallothionein gene MT-II and the newly discovered hemolysin gene HLP

Lachke, S. A., Srikantha, T., Tsai, L. K., Daniels, K. & Soll, D. R., 1 Feb 2000, In : Infection and Immunity. 68, 2, p. 884-895 12 p.

Research output: Contribution to journal › Article

Candida glabrata

Hemolysin Proteins

Metallothionein

Genes

DNA Fingerprinting

1999

46 Citations (Scopus)

Microevolutionary changes in Candida albicans identified by the complex Ca3 fingerprinting probe involve insertions and deletions of the full-length repetitive sequence RPS at specific genomic sites

Pujol, C., Joly, S., Nolan, B., Srikantha, T. & Soll, D. R., Oct 1999, In : Microbiology. 145, 10, p. 2635-2646 12 p.

Research output: Contribution to journal › Article

Nucleic Acid Repetitive Sequences

Candida albicans

Genetic Recombination

Genome

Sister Chromatid Exchange

151 Citations (Scopus)

Misexpression of the opaque-phase-specific gene PEP1 (SAP1) in the white phase of Candida albicans confers increased virulence in a mouse model of cutaneous infection

Kvaal, C., Lachke, S. A., Srikantha, T., Daniels, K., Mccoy, J. & Soll, D. R., 1 Dec 1999, In : Infection and Immunity. 67, 12, p. 6652-6662 11 p.

Research output: Contribution to journal › Article

Candida albicans

Virulence

Skin

Infection

Genes

1998

1 Citation (Scopus)

Advances in molecular genetics of Candida albicans and Candida glabrata

Brown, A. J. P., Cormack, B. P., Gow, N. A. R., Kvaal, C., Soll, D. R. & Thyagarajan, S., 1 Dec 1998, In : Journal of Medical and Veterinary Mycology. 3, p. 230-237 8 p.

Research output: Contribution to journal › Article

Candida glabrata

Candida albicans

Molecular Biology

Genetic Techniques

Candida

23 Citations (Scopus)

A MADS box protein consensus binding site is necessary and sufficient for activation of the opaque-phase-specific gene OP4 of Candida albicans

Lockhart, S. R., Nguyen, M., Thyagarajan, S. & Soll, D. R., 1 Dec 1998, In : Journal of Bacteriology. 180, 24, p. 6607-6616 10 p.

Research output: Contribution to journal › Article

Candida albicans

Protein Binding

Binding Sites

Genes

Transcriptional Activation

5 Citations (Scopus)

Reporters for the analysis of gene regulation in fungi pathogenic to man

Soll, D. R. & Thyagarajan, S., 1 Jan 1998, In : Current Opinion in Microbiology. 1, 4, p. 400-405 6 p.

Research output: Contribution to journal › Article

Reporter Genes

Fungi

Hyphae

Codon

Cues

82 Citations (Scopus)

The two-component hybrid kinase regulator CaNIK1 of Candida albicans

Srikantha, T., Tsai, L., Daniels, K., Enger, L., Highley, K. & Soll, D. R., Oct 1998, In : Microbiology. 144, 10, p. 2715-2729 15 p.

Research output: Contribution to journal › Article

Hyphae

Candida albicans

Phosphotransferases

Neurospora crassa

Histidine

1997

12 Citations (Scopus)

A novel repeat sequence (CKRS-1) containing a tandemly repeated sub-element (kre) accounts for differences between Candida krusei strains fingerprinted with the probe CkF1,2

Carlotti, A., Thyagarajan, S., Schröppel, K., Kvaal, C., Villard, J. & Soll, D. R., 1 Mar 1997, In : Current Genetics. 31, 3, p. 255-263 9 p.

Research output: Contribution to journal › Article

DNA Fingerprinting

Candida

Base Composition

Restriction Mapping

Intergenic DNA

96 Citations (Scopus)

Misexpression of the white-phase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence

Kvaal, C. A., Thyagarajan, S. & Soll, D. R., 7 Nov 1997, In : Infection and Immunity. 65, 11, p. 4468-4475 8 p.

Research output: Contribution to journal › Article

Candida albicans

Virulence

Genes

Phenotype

Open Reading Frames

22 Citations (Scopus)

The WH11 gene of Candida albicans is regulated in two distinct developmental programs through the same transcription activation sequences

Srikantha, T., Tsai, L. K. & Soll, D. R., Jun 1997, In : Journal of Bacteriology. 179, 12, p. 3837-3844 8 p.

Research output: Contribution to journal › Article

Renilla Luciferases

Hyphae

Candida albicans

Transcriptional Activation

Genes

1996

10 Citations (Scopus)

Cytoplasmic localization of the white phase-specific WH11 gene product of Candida albicans

Schröppel, K., Srikantha, T., Wessels, D., DeCock, M., Lockhart, S. R. & Soll, D. R., Aug 1996, In : Microbiology. 142, 8, p. 2245-2254 10 p.

Research output: Contribution to journal › Article

Candida albicans

Immune Sera

Genes

Cell Shape

Heat-Shock Proteins

126 Citations (Scopus)

The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans

Thyagarajan, S., Klapach, A., Lorenz, W. W., Tsai, L. K., Laughlin, L. A., Gorman, J. A. & Soll, D. R., 1 Jan 1996, In : Journal of Bacteriology. 178, 1, p. 121-129 9 p.

Research output: Contribution to journal › Article

Renilla Luciferases

Candida albicans

Reporter Genes

Gene Expression

Luciferases

1995

125 Citations (Scopus)

Colonizing populations of Candida albicans are clonal in origin but undergo microevolution through C1 fragment reorganization as demonstrated by DNA fingerprinting and C1 sequencing

Lockhart, S. R., Fritch, J. J., Meier, A. S., Schroppel, K., Thyagarajan, S., Galask, R. & Soll, D. R., 1 Jan 1995, In : Journal of Clinical Microbiology. 33, 6, p. 1501-1509 9 p.

Research output: Contribution to journal › Article

DNA Fingerprinting

Candida albicans

Population

Dermatoglyphics

Southern Blotting

47 Citations (Scopus)

Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans

Thyagarajan, S., Chandrasekhar, A. & Soll, D. R., 1 Jan 1995, In : Molecular and Cellular Biology. 15, 3, p. 1797-1805 9 p.

Research output: Contribution to journal › Article

Candida albicans

Genes

Firefly Luciferases

Trans-Activators

Transcriptional Activation

33 Citations (Scopus)

The frequency of integrative transformation at phase-specific genes of Candida albicans correlates with their transcriptional state

Thyagarajan, S., Morrow, B., Schröppel, K. & Soll, D. R., 1 May 1995, In : MGG Molecular & General Genetics. 246, 3, p. 342-352 11 p.

Research output: Contribution to journal › Article

Candida albicans

Spheroplasts

Genes

Phase Transition

Plasmids

1994

12 Citations (Scopus)

Developmental and molecular biology of switching in Candida albicans

Soll, D. R., Morrow, B., Srikantha, T., Vargas, K. & Wertz, P., Aug 1994, In : Oral Surgery, Oral Medicine, Oral Pathology. 78, 2, p. 194-201 8 p.

Research output: Contribution to journal › Article

Developmental Biology

Candida albicans

Molecular Biology

Genes

Virulence Factors

9 Citations (Scopus)

Partial nucleotide sequence of a single ribosomal RNA coding region and secondary structure of the large subunit 25 s rRNA of Candida albicans

Thyagarajan, S., Gutell, R. R., Morrow, B. & Soll, D. R., 1 Oct 1994, In : Current Genetics. 26, 4, p. 321-328 8 p.

Research output: Contribution to journal › Article

Ribosomal RNA

Ribosomal DNA

Candida albicans

Eukaryota

Yeasts

Srikantha Thyagarajan, PhD