Molecular regulation of cancer metastasis

Role of MIEN1 in cancer metastasis and EMT (epithelial-mesenchymal transition) in tumor progression

Interested in advancing therapeutic strategies for invasive cancer types

Colorectal cancer metastasis represents a substantial life threating malignancy. As per previous research, approximately 50% of colorectal cancer patients develop liver metastasis which is also an important cause of lethality. Migration and invasion enhancer1 (MIEN1) gene which was initially investigated as a key regulator of tumor cell migration and invasion and found to be overexpressed in different types of cancers such as breast cancer, ovarian cancer, prostate cancer, gastric cancer, nonsmall cell lung cancer. However, MIEN-1’s role in the colorectal cancer metastasis is still unclear and requires further elucidation for the development of potential therapeutics. My research is more focused to examine MIEN1’s role in cancer metastasis. We are aimed to understand the role of MIEN1 in metastatic colorectal cancer and EMT progression.

Molecular biological techniques and technical skills

• Western blotting
• Agarose Gel Electrophoresis
• Real-time PCR
• ELISA
• Fluorescence microscopy
• Immunohistochemistry
• Micro CT scan and CLAMS
• DNA, RNA, protein isolation methods
• Toxicity determination (MTT assay)
• CRISPR (gene editing)
• Immunoprecipitation
• SiRNA transfection
• Cell viability Assay

Animal Handling (in-vivo mice and rats) and related experiments

• In Vivo Research: Skilled in rodent disease models, dosing routes, anesthesia, necropsy, and tissue processing.
• Data Analysis & Tools: Proficient in Excel, GraphPad Prism, ImageJ, and data analysis tools.
• Molecular Biology Techniques: Extensive experience in flow cytometry, ELISA, qPCR, and tissue analysis.
• Collaborative Research: Strong ability to collaborate with cross-functional teams in study design, data presentation, and research execution.

Experimental Optimization: Experience optimizing experimental workflows and assay performance for increased efficiency and robustness