Research output per year
Research output per year
Personal profile
Research Interests
Molecular regulation of cancer metastasis
Role of MIEN1 in cancer metastasis and EMT (epithelial-mesenchymal transition) in tumor progression
Interested in advancing therapeutic strategies for invasive cancer types
Area of Expertise
Colorectal cancer metastasis represents a substantial life threating malignancy. As per previous research, approximately 50% of colorectal cancer patients develop liver metastasis which is also an important cause of lethality. Migration and invasion enhancer1 (MIEN1) gene which was initially investigated as a key regulator of tumor cell migration and invasion and found to be overexpressed in different types of cancers such as breast cancer, ovarian cancer, prostate cancer, gastric cancer, nonsmall cell lung cancer. However, MIEN-1’s role in the colorectal cancer metastasis is still unclear and requires further elucidation for the development of potential therapeutics. My research is more focused to examine MIEN1’s role in cancer metastasis. We are aimed to understand the role of MIEN1 in metastatic colorectal cancer and EMT progression.
Techniques
Molecular biological techniques and technical skills
- Western blotting
- Agarose Gel Electrophoresis
- Real-time PCR
- ELISA
- Fluorescence microscopy
- Immunohistochemistry
- Micro CT scan and CLAMS
- DNA, RNA, protein isolation methods
- Toxicity determination (MTT assay)
- CRISPR (gene editing)
- Immunoprecipitation
- SiRNA transfection
- Cell viability Assay
Animal Handling (in-vivo mice and rats) and related experiments
- In Vivo Research: Skilled in rodent disease models, dosing routes, anesthesia, necropsy, and tissue processing.
- Data Analysis & Tools: Proficient in Excel, GraphPad Prism, ImageJ, and data analysis tools.
- Molecular Biology Techniques: Extensive experience in flow cytometry, ELISA, qPCR, and tissue analysis.
- Collaborative Research: Strong ability to collaborate with cross-functional teams in study design, data presentation, and research execution.
Experimental Optimization: Experience optimizing experimental workflows and assay performance for increased efficiency and robustness
Education/Academic qualification
B.Sc. in Zoology, Banaras Hindu University, Varanasi
M.Sc. in Department of Biotechnology, Kumaun University India
Ph.D. in CSIR-Central Drug Research Institute , University of Lucknow
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Collaborations and top research areas from the last five years
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Systemic Insulin Resistance and Metabolic Perturbations in Chow Fed Inducible Nitric Oxide Synthase Knockout Male Mice: Partial Reversal by Nitrite Supplementation
Aggarwal, H., Pathak, P., Singh, P., Gayen, J., Jagavelu, K. & Dikshit, M., 12 Aug 2020, In: Antioxidants.Research output: Contribution to journal › Article › peer-review
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Evaluation of mutagenic, cytotoxic, mitochondrial dysfunction, apoptotic activity, and acute toxicity of ethanolic extract of Cissus quadrangularis
Syed, A. A., Reza, M. I., Kalleti, N., Husain, A., Singh, P., Kumar Rath, S. & Gayen, J., 1 Jan 2023, In: Toxicology. 483Research output: Contribution to journal › Article › peer-review
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Emerging nanotechnology-based combination therapies of taxanes for multiple drug-resistant cancers
Kater, R., Singh, P., Garg, R., Verma, S. & Gayen, J., 5 Dec 2021, In: Pharmaceutical Development and Technology. p. 95-107Research output: Contribution to journal › Article › peer-review
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Ethanolic extract of Cissus quadrangularis improves vasoreactivity by modulation of eNOS expression and oxidative stress in spontaneously hypertensive rats
Syed, A. A., Shafiq, M., Reza, M. I., Bharati, P., Singh, P., Hanif, K. & Gayen, J., 2 Jan 2022, In: Clinical and Experimental Hypertension. 44, 1, p. 63-71Research output: Contribution to journal › Article › peer-review
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Herb–drug interaction studies of ethanolic extract of Cassia occidentalis L. coadministered with acetaminophen, theophylline, omeprazole, methotrexate, and methylprednisolone
Husain, A., Riyazuddin, M., Katekar, R., Verma, S., Syed, A. A., Singh, P., Chattopadhyay, N. & Gayen, J., Feb 2021, In: Phytomedicine. 1, 1Research output: Contribution to journal › Article › peer-review