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Personal profile

Area of Expertise

My interest is to understand the molecular mechanism responsible for contraction of human ventricles. It is known that contraction is caused by the interaction between two major muscle proteins (actin and myosin), and that it involves the rolling motion of the myosin molecule on a “rail” of actin molecules, but the details of this process are unknown. I believe this process cannot be studied using an isolated cardiac myocyte because it is too large: it contains billions of myosin molecules. Averaging the signal originating from so many molecules erases any information about the dynamics of molecular motion. To understand molecular motion one must look at a single myosin molecule within a myocyte. This requires a special technique. I have been using a technique called Surface Plasmon Assisted Emission (SPAM - developed at this university), which greatly reduces the background signal from molecules other than myosin. I have built a microscope based on SPAM to investigate the motion of myosin in cardiac myocytes from human ventricles. The results provide specific information about the dynamics of the rolling motion of cardiac myosin.

Education/Academic qualification

PhD in Biophysics, Macquarie University

Fingerprint Dive into the research topics where Julian Borejdo is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 3 Similar Profiles
Myosins Medicine & Life Sciences
Actins Medicine & Life Sciences
Myosin Subfragments Medicine & Life Sciences
Myofibrils Medicine & Life Sciences
Fluorescence Chemical Compounds
Muscle Chemical Compounds
Muscles Medicine & Life Sciences
fluorescence Physics & Astronomy

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Projects 2010 2015

Research Output 1974 2018

1 Citation (Scopus)

Differences in the spatial distribution of actin in the left and right ventricles of functioning rabbit hearts

Nagwekar, J., Duggal, D., Rich, R., Fudala, R., Gryczynski, I., Raut, S. L., Gryczynski, Z. & Borejdo, J., 1 Mar 2018, In : Medical Photonics. 27, p. 1-8 8 p.

Research output: Contribution to journalArticle

Heart Ventricles
Actins
Myosins
Rabbits
Muscles

Surface plasmon-assisted microscope

Borejdo, J., Gryczynski, Z., Fudala, R., Joshi, C. R., Borgmann, K. R., Ghorpade, A. & Gryczynski, I., 1 Jun 2018, In : Journal of Biomedical Optics. 23, 6, 060502.

Research output: Contribution to journalArticle

Microscopes
microscopes
membranes
metal surfaces
Metals
2 Citations (Scopus)

Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells

Requena, S., Ponomarchuk, O., Castillo, M., Rebik, J., Brochiero, E., Borejdo, J., Gryczynski, I., Dzyuba, S. V., Gryczynski, Z., Grygorczyk, R. & Fudala, R., 1 Dec 2017, In : Scientific Reports. 7, 1, 16761.

Research output: Contribution to journalArticle

Mucins
Cystic Fibrosis
Viscosity
Optical Imaging
Mucus

No difference in myosin kinetics and spatial distribution of the lever arm in the left and right ventricles of human hearts

Duggal, D., Requena, S., Nagwekar, J., Raut, S. L., Rich, R., Das, H., Patel, V., Gryczynski, I., Fudala, R., Gryczynski, Z., Blair, C., Campbell, K. S. & Borejdo, J., 13 Oct 2017, In : Frontiers in Physiology. 8, OCT, 732.

Research output: Contribution to journalArticle

Myosins
Heart Ventricles
Muscle Cells
Ventricular Myosins
Muscle Proteins
2 Citations (Scopus)

Demonstration of FRET in solutions

Shah, S., Gryczynski, Z., Chib, R., Fudala, R., Baxi, A., Borejdo, J., Synak, A. & Gryczynski, I., Mar 2016, In : Methods and Applications in Fluorescence. 4, 1, 015001.

Research output: Contribution to journalArticle

Rhodamines
rhodamine
Energy transfer
Demonstrations
Fluorescence