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Personal profile

Area of Expertise

My research interests cover fluorescence spectroscopy and its applications in biochemistry and biology. I am developing advanced fluorescence-based techniques for ultra-sensitive detection of analytes and biomedical agents. In particular, we have developed a FRET-based method for the selective detection of a metalloproteinase 9 enzyme (MMP-9) and micro RNAs. The enhanced detection of minute amounts of dye molecules has been achieved with a Metal Enhanced Fluorescence (MEF). Several plasmonic platforms have been developed and tested for MEF. These include silver island films, silver fractals, and silver nanowires deposited on glass/sapphire substrates. We demonstrated several hundredfold enhancements of the fluorescence signals by using our plasmonic platforms. We also applied surface plasmon coupled emission (SPCE) in fluorescence microscopy. SPCE transforms an isotropic fluorescence emission into a directional, fully polarized radiation. The SPCE radiation preserves all spectral properties of used fluorophores and offers a significant reduction of unwanted fluorescence background because of minimization of the excitation volume.

Education/Academic qualification

PhD in Physics, University of Gdansk

MS in Physics, University of Gdansk

Fingerprint Dive into the research topics where Ignacy Gryczynski is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 4 Similar Profiles
Fluorescence Chemical Compounds
fluorescence Physics & Astronomy
Fluorophores Chemical Compounds
Silver Chemical Compounds
Anisotropy Chemical Compounds
Energy transfer Chemical Compounds
Photons Chemical Compounds
Quenching Chemical Compounds

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Projects 2009 2016

Research Output 1975 2019

Non-fluorescent filters for fluorescence detection with in-line geometry

Kimball, J., Chavez, J., Ceresa, L., Shah, S., Gryczynski, I. & Gryczynski, Z., 16 May 2019, In : Methods and Applications in Fluorescence. 7, 3, 037001.

Research output: Contribution to journalArticleResearchpeer-review

Potassium Dichromate
lines (geometry)
chromates
Potassium
Fluorescence

Photophysical properties and detection of Valrubicin on plasmonic platforms

Synak, A., Szczepańska, E., Grobelna, B., Gondek, J., Mońka, M., Gryczynski, I. & Bojarski, P., 1 Apr 2019, In : Dyes and Pigments. 163, p. 623-627 5 p.

Research output: Contribution to journalArticleResearchpeer-review

valrubicin
Silver
Nanoparticles
Plasmons
Gold

Spectroscopic method for estimation of MMP-9 enzyme concentration and activity

Synak, A., Serdiuk, I. E., Grobelna, B., Fudala, R., Gryczynski, I. & Bojarski, P., 15 Jul 2019, In : Journal of Molecular Liquids. 286, 110936.

Research output: Contribution to journalArticleResearchpeer-review

Matrix Metalloproteinases
Peptides
peptides
enzymes
Enzymes
1 Citation (Scopus)

AMCA to TAMRA long range resonance energy transfer on a flexible peptide

Synak, A., Fudala, R., Gryczynski, I., Kułak, L., Shah, S., Serdiuk, I. E., Grobelna, B., Arłukowicz, P., Kubicki, A. & Bojarski, P., 1 Nov 2018, In : Dyes and Pigments. 158, p. 60-64 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Tranexamic Acid
Energy transfer
Peptides
Fluorescence
Glycerol
1 Citation (Scopus)

Differences in the spatial distribution of actin in the left and right ventricles of functioning rabbit hearts

Nagwekar, J., Duggal, D., Rich, R., Fudala, R., Gryczynski, I., Raut, S. L., Gryczynski, Z. & Borejdo, J., 1 Mar 2018, In : Medical Photonics. 27, p. 1-8 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Heart Ventricles
Actins
Myosins
Rabbits
Muscles