• 14220 Citations
  • 57 h-Index
1975 …2020

Research output per year

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Personal profile

Area of Expertise

My research interests cover fluorescence spectroscopy and its applications in biochemistry and biology. I am developing advanced fluorescence-based techniques for ultra-sensitive detection of analytes and biomedical agents. In particular, we have developed a FRET-based method for the selective detection of a metalloproteinase 9 enzyme (MMP-9) and micro RNAs. The enhanced detection of minute amounts of dye molecules has been achieved with a Metal Enhanced Fluorescence (MEF). Several plasmonic platforms have been developed and tested for MEF. These include silver island films, silver fractals, and silver nanowires deposited on glass/sapphire substrates. We demonstrated several hundredfold enhancements of the fluorescence signals by using our plasmonic platforms. We also applied surface plasmon coupled emission (SPCE) in fluorescence microscopy. SPCE transforms an isotropic fluorescence emission into a directional, fully polarized radiation. The SPCE radiation preserves all spectral properties of used fluorophores and offers a significant reduction of unwanted fluorescence background because of minimization of the excitation volume.

Education/Academic qualification

MS in Physics, University of Gdańsk

PhD in Physics, University of Gdańsk

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Projects

Research Output

On the origin and correction for inner filter effects in fluorescence Part I: primary inner filter effect-the proper approach for sample absorbance correction

Kimball, J., Chavez, J., Ceresa, L., Kitchner, E., Nurekeyev, Z., Doan, H., Szabelski, M., Borejdo, J., Gryczynski, I. & Gryczynski, Z., 1 Jun 2020, In : Methods and Applications in Fluorescence. 8, 3, 1 p.

Research output: Contribution to journalArticle

  • On the possibility of direct triplet state excitation of indole

    Chavez, J., Ceresa, L., Kitchner, E., Kimball, J., Shtoyko, T., Fudala, R., Borejdo, J., Gryczynski, Z. & Gryczynski, I., Jul 2020, In : Journal of Photochemistry and Photobiology B: Biology. 208, 111897.

    Research output: Contribution to journalArticle

  • Probing the assembly of HDL mimetic, drug carrying nanoparticles using intrinsic fluorescences

    Raut, S., Garud, A., Nagarajan, B., Sabnis, N., Remaley, A., Fudala, R., Gryczynski, I., Gryczynski, Z., Dzyuba, S. V., Borejdo, J. & Lacko, A., 1 Apr 2020, In : Journal of Pharmacology and Experimental Therapeutics. 373, 1, p. 113-121 9 p.

    Research output: Contribution to journalArticle

    Open Access
  • Fluorescence anisotropy imaging in drug discovery

    Vinegoni, C., Feruglio, P. F., Gryczynski, I., Mazitschek, R. & Weissleder, R., 1 Nov 2019, In : Advanced Drug Delivery Reviews. 151-152, p. 262-288 27 p.

    Research output: Contribution to journalReview article

  • 8 Scopus citations